BAC Libraries for the Reptilia, Including Birds: Genomic Resources for Comparative Biology

BAC 爬虫类（包括鸟类）文库：比较生物学的基因组资源

• 批准号: 0431717
• 负责人: Scott Edwards
• 金额: $ 71.51万
• 依托单位: Harvard University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-02-01 至 2005-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0431717&HistoricalAwards=false
• 关键词: BAC; Libraries; Reptilia; Including; Birds

0207870EdwardsBacterial artificial chromosome libraries are collections of large (100, 000 - 200,000 DNA base pair) DNA fragments that are made retrievable and manipulatable by insertion into a specialized vector. As resources for easily obtaining large amounts of DNA sequences for comparative purposes, for understanding the structure of eukaryotic genomes, and for expressing and manipulating genes and their phenotypic effects in developing embryos, BAC libraries are unsurpassed. However, constructing BAC libraries is beyond the abilities of most laboratories not specifically tailored for this purpose. This project will consist of constructing, archiving and disseminating to the international community of scientists BAC libraries from five species of Reptilia, including a bird. The five focal species will be Painted Turtle (Chrysemys picta), Tuatara (Sphenodon punctatus), South American Worm Lizard (an amphisbaenian; Amphisbaea alba), American Alligator (Alligator mississippiensis), and the flightless Emu (Dromaius novaehollandiae). In the event that an appropriate Tuatara sample is not available (a distinct possibility given its endangered status and restricted range in New Zealand), a BAC library from a snake will be constructed. Reptilia, consisting of birds as well as species commonly referred to as reptiles, are poorly studied from genetic, developmental, genomic and phylogenetic perspectives; although Reptilia are much more diverse than their sister group, the mammals, mammals are much better studied as model biological species. The construction and arraying of BAC libraries from these species will open up many new avenues of research into reptile genomics that were previously unapproachable. There will be many scientific uses for these BAC libraries, including facilitating acquisition of comparative DNA data for phylogenetic and comparative genomic purposes, and a better understanding of the function of genes responsible for many features first appearing in the reptiles, such as feathers, temperature-dependent sex determination, limb reduction and loss, and large-scale genome reduction compared to mammals. Thus these libraries will fill a major gap in our understanding of vertebrate genomics, a rapidly progressing discipline spurred on by the recent sequencing of the complete human, mouse and pufferfish genomes.

0207870Edwardsbacterial人造染色体库是大型（100，000-200,000 DNA碱基对）的DNA片段的集合，可通过将其插入专门向量进行检索和操纵。 作为用于比较目的的大量DNA序列的资源，以理解真核基因组的结构，以及表达和操纵基因及其在开发胚胎中的表型效应，BAC库是无与伦比的。 但是，构建BAC库超出了大多数不专门为此目的量身定制的实验室的能力。 该项目将包括构建，存档和传播给来自五种爬行动物（包括鸟）的国际科学家BAC图书馆社会。 这五个焦点将被涂成乌龟（Chrysemys Picta），Tuatara（Sphenodon Punctatus），南美蠕虫蜥蜴（Amphisbaenian; Amphisbaea alba），美国鳄鱼（Almphisbaea alba），美国鳄鱼（Alligator Mississipsippiensis）和无飞行Emu（Dromaius novaeaehollandiae）。 如果无法获得适当的Tuatara样品（鉴于其濒临灭绝的状态和新西兰的限制范围有明显的可能性），将构建来自蛇的BAC库。 爬行动物由鸟类以及通常称为爬行动物的物种组成，是从遗传，发育，基因组和系统发育的观点中研究的。尽管爬行动物比他们的姐妹组更多样化，但哺乳动物的研究要好得多。 这些物种的BAC库的构建和阵列将为爬行动物基因组学的许多新途径开放，这些研究以前是不可接受的。 这些BAC库将有许多科学用途，包括促进对比较DNA数据的获取，以实现系统发育和比较基因组目的，并更好地理解负责首先出现在爬行动物中的许多特征的基因的功能，例如羽毛，诸如温度依赖性的性别确定，肢体降低和降低和损失，以及较大的基因组相比。 因此，这些文库将填补我们对脊椎动物基因组学的理解，这是一个迅速发展的纪律，这是由于最近对完整的人，小鼠和pufferfish基因组的测序所刺激的。