CAREER: Nanofluidic Molecular Filters

职业：纳米流体分子过滤器

基本信息

批准号：
0347348
负责人：
Jongyoon Han
金额：
$ 40万
依托单位：
Massachusetts Institute of Technology
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2004
资助国家：
美国
起止时间：
2004-01-15 至 2009-12-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0347348&HistoricalAwards=false
关键词：
CAREER Nanofluidic Molecular Filters

项目摘要

AbstractProposal Title: CAREER: Nanofluidic Molecular FiltersProposal Number: CTS-0347348Principal Investigator: Jongyoon HanInstitution: Massachusetts Institute of TechnologyThe aim of the proposed research is to develop new micromachined nanofluidic filters for biomolecules. Specific goals include fabrication and characterization of nanofluidic filters, demonstration of molecular-size sieving mechanism, and a study of the molecular charge sieving. Understanding of biomolecule interactions with nanopores is essential for optimization and improvement of molecular separation and filtering. The proposed work will address this important issue by studying biomolecular separations in nanofluidic channels with well-defined size and shape as well as surface charge. The details of the program are well thought out. The fabrication of the nanofluidic channels with varying depths will be achieved by patterning and etching silicon wafers. The channels will be characterized by studying the dynamics of various DNA molecules and comparing the results with previous studies. The PI outlines potential collaborative opportunities with other research groups at MIT, namely those that have expertise in areas such as confocal/two-photon microscopy and fluorescence correlation microscopy. Nanofluidic filters represent a novel approach for biomolecular separations. In addition to potential practical applications, this technology will impact our understanding of molecular interaction and dynamics within nanostructures. The educational aspects of this program include the incorporation of biological engineering research problems in existing MIT courses, development of a Molecular Movies website covering molecular transport and diffusion, and the development of a new lab module that covers biomolecule transport mechanisms. The educational program will propel both undergraduate and graduate education to new frontiers in biological engineering.

摘要提案标题：职业：纳米流体分子过滤器提案编号：CTS-0347348 首席研究员：Jongyoon Han 机构：麻省理工学院该研究的目的是开发用于生物分子的新型微机械纳米流体过滤器。具体目标包括纳米流体过滤器的制造和表征、分子尺寸筛分机制的演示以及分子电荷筛分的研究。了解生物分子与纳米孔的相互作用对于优化和改进分子分离和过滤至关重要。拟议的工作将通过研究具有明确尺寸和形状以及表面电荷的纳米流体通道中的生物分子分离来解决这一重要问题。该计划的细节经过深思熟虑。不同深度的纳米流体通道的制造将通过图案化和蚀刻硅片来实现。这些通道将通过研究各种 DNA 分子的动力学并将结果与之前的研究进行比较来表征。该 PI 概述了与麻省理工学院其他研究小组的潜在合作机会，即那些在共焦/双光子显微镜和荧光相关显微镜等领域拥有专业知识的研究小组。纳流体过滤器代表了一种生物分子分离的新方法。除了潜在的实际应用之外，这项技术还将影响我们对纳米结构内分子相互作用和动力学的理解。该计划的教育方面包括将生物工程研究问题纳入现有的麻省理工学院课程，开发涵盖分子运输和扩散的分子电影网站，以及开发涵盖生物分子运输机制的新实验室模块。该教育计划将推动本科生和研究生教育迈向生物工程的新领域。