Second Harmonic Ellipsometry and Spectroscopy of Biological Interfaces

生物界面的二次谐波椭圆测量和光谱学

• 批准号: 0316177
• 负责人: Garth Simpson
• 金额: $ 31.9万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0316177&HistoricalAwards=false
• 关键词: Second; Harmonic; Ellipsometry; Spectroscopy; Biological

In this project, funded by the Experimental Physical Chemistry Program of the Chemistry Division, Prof. Garth Simpson of Purdue Universty will investigate the chirality of biological molecules on surfaces using nonlinear optical techniques based on surface second harmonic (SHG) generation and sum frequency generation (SFG). SHG polarization measurements are by several orders of magnitude more sensitive to interfacial chirality than traditional absorbance-based methods. The goal of this research is to gain a fundamental understanding of the nonlinear optical properties of biological interfaces and thereby establish a foundation for interpreting future studies of such systems. The research component of this project consists of three specific tasks: 1) to elucidate the mechanisms responsible for the large chiral effects in nonlinear optical measurements of ordered chiral films; 2) to develop nonlinear optical null ellipsometry as a novel and robust approach for acquiring and interpreting nonlinear polarization measurements of oriented chiral and achiral surface systems; and 3) to make nonlinear optical measurements of model lipid and proteolipid interfaces to provide a context in which to interpret nonlinear optical measurements of more complex biological systems. Graduate and undergraduate students as well as postdoctoral research associates will participate in the research.In addition to these fundamental investigations Prof. Simpson will develop laser instrumentation modules suitable for use in K-12 Education through the Purdue University Science Bound pilot program. This program targets 7th through 12th grade students in the Indianapolis Public Schools by means of three components: 1) after school programs; 2) follow-up activities; and 3) mentor teacher professional development.

在该项目中，由化学系实验物理化学项目资助，普渡大学 Garth Simpson 教授将利用基于表面二次谐波 (SHG) 生成和和频生成的非线性光学技术来研究表面生物分子的手性。 SFG）。 相比传统的基于吸光度的方法，二次谐波偏振测量对界面手性的敏感度高出几个数量级。 这项研究的目标是获得对生物界面非线性光学特性的基本了解，从而为解释此类系统的未来研究奠定基础。 该项目的研究部分包括三个具体任务：1）阐明有序手性薄膜非线性光学测量中大手性效应的机制； 2）开发非线性光学零椭偏仪作为一种新颖且稳健的方法，用于获取和解释定向手性和非手性表面系统的非线性偏振测量； 3) 对模型脂质和蛋白脂质界面进行非线性光学测量，以提供解释更复杂生物系统的非线性光学测量的背景。 研究生和本科生以及博士后研究助理将参与这项研究。除了这些基础研究之外，辛普森教授还将通过普渡大学科学边界试点计划开发适用于 K-12 教育的激光仪器模块。 该计划针对印第安纳波利斯公立学校 7 至 12 年级的学生，分为三个部分：1) 课后计划； 2）后续活动； 3) 指导教师专业发展。