MRI: Track 1 Development of a Dual-Comb Fluorescence-Detected Photothermal Mid-Infrared (DC-FPTIR) Microscope

MRI：轨道 1 双梳荧光检测光热中红外 (DC-FPTIR) 显微镜的开发

• 批准号: 2320751
• 负责人: Garth Simpson
• 金额: $ 73.74万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2320751&HistoricalAwards=false
• 关键词: MRI; Track; Development; Dual; Comb

This award is jointly supported by the Major Research Instrumentation (MRI), the Chemistry Research Instrumentation, and Chemical Measurement and Imaging (CMI) programs. Purdue University is developing a novel fluorescence-detected photothermal microscope using a dual-comb laser source to support the research of Professor Garth Simpson along with colleagues Yoon Yeo, Lynn Tayler, Arezoo Ardekani, and Paul Schmitt. This instrument facilitates research in the areas of nanoparticle and advanced materials analysis, fundamental studies of biological systems, and measurement of molecular composition in plants and animals. The instrument enables high-resolution optical microscopy with contrast arising from specific molecular bonds. In general, dual-comb spectroscopy uses high frequency optical signals to produce radio-frequency beat patterns that can be detected by high-speed digitization. These beat patterns modulate the local temperature and the corresponding fluorescence intensity. Infrared spectroscopy is then performed with a spatial resolution set by fluorescence microscopy and IR-fluorescence colocalization dependent on the local thermal conductivity. In addition to supporting fundamental science directly, this award targets the enhancement of educational, research, and research training at all levels in multiple departments. This developed instrument is also used to benefit activities within the NSF-supported Center for Bioanalytic Metrology, an NSF Research Experience for Undergraduates (REU) program, and major outreach and educational activities of the newly established Young Institute for Advanced Manufacturing of Pharmaceuticals. This award is aimed at enhancing research and education at all levels. Specifically, the development of this instrument offers the unique ability to perform the spectroscopy characterization of therapeutic nanoparticle transformations. The instrumentation enables chemical mapping within phase separated domains affecting dissolution in therapeutic materials. By using the dual-comb laser source, this instrument also aids in the structural elucidation and compositional changes during virus maturation.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项由主要研究仪器（MRI）、化学研究仪器和化学测量与成像（CMI）项目联合支持。普渡大学正在开发一种使用双梳激光源的新型荧光检测光热显微镜，以支持 Garth Simpson 教授及其同事 Yoon Yeo、Lynn Tayler、Arezoo Ardekani 和 Paul Schmitt 的研究。 该仪器促进了纳米粒子和先进材料分析、生物系统基础研究以及动植物分子组成测量等领域的研究。该仪器可实现高分辨率光学显微镜，并具有由特定分子键产生的对比度。一般来说，双梳光谱学使用高频光信号来产生可以通过高速数字化检测的射频拍频模式。这些节拍模式调节局部温度和相应的荧光强度。 然后，根据局部热导率，以荧光显微镜和红外荧光共定位设定的空间分辨率进行红外光谱分析。 除了直接支持基础科学外，该奖项还旨在加强多个部门各级的教育、研究和研究培训。 该开发的仪器还用于促进 NSF 支持的生物分析计量中心的活动、NSF 本科生研究体验 (REU) 计划以及新成立的青年先进药品制造研究所的主要外展和教育活动。该奖项旨在加强各级研究和教育。 具体来说，该仪器的开发提供了执行治疗性纳米粒子转化的光谱表征的独特能力。 该仪器能够在影响治疗材料溶解的相分离域内进行化学绘图。 通过使用双梳激光源，该仪器还有助于病毒成熟过程中的结构阐明和成分变化。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Theoretical basis for interpreting heterodyne chirality-selective sum frequency generation spectra of water

解释水的外差手性-选择和频率产生谱的理论基础

• 发表时间: 2024-02
• 期刊: The Journal of chemical physics
• 作者: Konstantinovsky, Daniel;Santiago, Ty;Tremblay, Matthew;Simpson, Garth J;Hammes;Yan, Elsa C
• 通讯作者: Yan, Elsa C