Deciphering Mechanisms of Speciation Among Host-Symbiont Populations

破译宿主共生群体之间的物种形成机制

• 批准号: 0316516
• 负责人: Michele Nishiguchi
• 金额: $ 38.7万
• 依托单位: New Mexico State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0316516&HistoricalAwards=false
• 关键词: Deciphering; Mechanisms; Speciation; Among; Host

The mutualistic association between sepiolid squids (Mollusca: Cephalopoda) and their luminous bacterial symbionts (Genus Vibrio) provides a versatile model system to study population biology and speciation among animal-bacterial partnerships. Although the squid light organs are open to the seawater, only two Vibrio species stay inside the organ, indicating adaptation and evolution of specificity. Using state-of-the-art techniques involving fluorescent microscopy, molecular biology, and gene expression, this project will examine whether environmental processes or the squid hosts determine the architecture of these interactions. Central to this study will be determining how different the squid-Vibrio pairs are among geographically isolated populations. A key element of this proposal is the several international and collaborative efforts that have been established by the Nishiguchi laboratory for this work. Results from this proposal will be shared (through publications, meetings, web sites, courses) with both the research and public communities. This study will provide unique research opportunities for students, building on programs for under-represented minorities at New Mexico State University. NMSU is the only Carnegie I research institution ranked by the National Science Foundation as one that serves significant numbers of two ethnic minority student populations, Hispanics and Native Americans. This research will lead to a better understanding of speciation and evolution of bacterial-animal relationships and provide information about the transmission of bacteria in the environment.

海泡乌贼（软体动物：头足类）与其发光细菌共生体（弧菌属）之间的互惠关系为研究种群生物学和动物-细菌伙伴关系之间的物种形成提供了一个通用的模型系统。 尽管乌贼的发光器官向海水开放，但只有两种弧菌留在该器官内，这表明了其适应和进化的特异性。 该项目将利用涉及荧光显微镜、分子生物学和基因表达的最先进技术，研究环境过程或鱿鱼宿主是否决定了这些相互作用的结构。 这项研究的核心是确定地理上孤立的乌贼-弧菌对之间的差异有多大。 该提案的一个关键要素是西口实验室为此工作建立的多项国际合作努力。该提案的结果将与研究和公共社区共享（通过出版物、会议、网站、课程）。 这项研究将以新墨西哥州立大学针对代表性不足的少数群体的项目为基础，为学生提供独特的研究机会。 NMSU 是唯一一所被美国国家科学基金会评为为大量西班牙裔和美洲原住民这两个少数族裔学生群体提供服务的卡内基 I 研究机构。 这项研究将有助于更好地了解细菌与动物关系的物种形成和进化，并提供有关细菌在环境中传播的信息。