Discovery and Characterization of Bacterial Endosymbiont Diversity in Drosophila

果蝇细菌内共生多样性的发现和表征

• 批准号: 0315815
• 负责人: Nancy Moran
• 金额: --
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-12-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0315815&HistoricalAwards=false
• 关键词: Discovery; Characterization; Bacterial; Endosymbiont; Diversity

DEB-0315815 (Abstract)"Discovery and Characterization of Bacterial Endosymbiont Diversity in Drosophila"A grant has been awarded to Dr Nancy Moran and Dr. Therese Markow of the Universityof Arizona to use molecular methods to document the diversity of bacterial symbionts that form chronic infections in species of fruitflies (genus Drosophila and relatives). A primary aim is to use DNA sequences as well as microscopy to characterize the diversity and distributions of bacteria infecting an evolutionarily related set of host species. Such information is so far not available for any animal group. Results will illuminate how genetic divergence, geographic separation, or ecological differences of hosts affect infection by particular bacterial types. The data will be made widely available to researchers on a website and also through public DNA sequence databases. Increasing evidence shows that bacterial infection is usual even in healthy individuals of all animal species, from insects to humans, but the diversity of bacteria associated with particular host species or groups of related species is unexplored. This project will provide such information for fruitfly species, for which there exists a wealth of information on genetics and evolutionary relationships, including a completed genome sequence. Knowledge of the bacteria infecting fruitflies will provide study systems for a large community of researchers spanning the fields of genetics, infectious disease, and ecology. Once characterized, these symbiotic bacteria will be useful for addressing such questions as how chronic infections interact with host defenses at the molecular level and how infectious agents move between host species, sometimes resulting in the emergence of new diseases in humans and other species. Insects, including crop pests and disease vectors, frequently harbor symbionts, which have been shown to affect reproduction and vectoring capabilities of their hosts. Results of this research will aid attempts to control insect pests by manipulating symbiont associations.

deb-0315815（摘要）“果蝇中细菌内共生多样性的发现和表征”已向亚利桑那大学的南希·莫兰博士和特雷斯·马克博士授予了赠款在果蝇（果蝇属和亲戚）中。主要目的是使用DNA序列以及显微镜来表征感染一组与进化相关的宿主物种的细菌的多样性和分布。到目前为止，任何动物群都无法使用此类信息。结果将阐明遗传差异，地理分离或宿主的生态差异如何影响特定细菌类型的感染。数据将在网站上以及通过公共DNA序列数据库中广泛使用。越来越多的证据表明，即使在从昆虫到人类的所有动物物种的健康个体中，细菌感染也通常是通常的，但是与特定宿主物种或相关物种组相关的细菌的多样性尚未探索。该项目将为果蝇物种提供此类信息，其中存在有关遗传学和进化关系的大量信息，包括完整的基因组序列。感染水果的细菌的知识将为跨越遗传学，传染病和生态学领域的大量研究人员提供研究系统。一旦表征，这些共生细菌将有助于解决诸如慢性感染如何与分子水平与宿主防御相互作用的问题以及传染剂在宿主物种之间的移动，有时会导致人类和其他物种的新疾病出现。昆虫，包括作物害虫和疾病媒介，经常藏有共生体，这些昆虫已证明会影响宿主的繁殖和矢量功能。这项研究的结果将通过操纵共生体关联来帮助控制害虫。