Development of Biodiagnostic Devices based on Differential Adhesion Mediated by DNA Hybridization

基于 DNA 杂交介导的差异粘附的生物诊断装置的开发

• 批准号: 0314265
• 负责人: Daniel Hammer
• 金额: $ 45.12万
• 依托单位: University of Pennsylvania
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-15 至 2007-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0314265&HistoricalAwards=false
• 关键词: Development; Biodiagnostic; Devices; based; Differential

The objective of this project is to develop and optimize methods for the detection of unknown DNA strands by the adhesion of micro-sized particles bearing DNA to surfaces bearing complementary strands of DNA. Such techniques would combine DNA hybridization as a high fidelity means of identifying unknown strands, with bead based adhesion, in which the large number of complementary strands leads to a higher signal-to-noise in DNA detection, as well as a sharper dependence on external variables that affect binding, such as temperature, molecular density and flow rate. Furthermore, the use of micro-sized particles would lead to simple optical detection of binding, which would facilitate and broaden the methods for detection. The methods developed here will be useful for the detection of foreign DNA from pathogenic bacteria, and thus be useful for pathogen detection and the national defense against bioterrorism.

该项目的目标是开发和优化通过将带有 DNA 的微米颗粒粘附到带有互补 DNA 链的表面来检测未知 DNA 链的方法。此类技术将 DNA 杂交作为识别未知链的高保真方法与基于珠子的粘附结合起来，其中大量的互补链导致 DNA 检测中的信噪比更高，并且对外部的依赖更加强烈。影响结合的变量，例如温度、分子密度和流速。此外，微米尺寸颗粒的使用将导致结合的简单光学检测，这将促进和拓宽检测方法。这里开发的方法将有助于检测病原菌中的外源DNA，从而有助于病原体检测和防御生物恐怖主义。