DISSERTATION RESEARCH: Systematics of Carex Section Ovales: The Challenge of Phylogeny at Fine Scales

论文研究：卵形苔草节的系统学：精细尺度系统发育的挑战

• 批准号: 0308975
• 负责人: Paul Berry
• 金额: $ 0.99万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-15 至 2005-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0308975&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Systematics; Carex; Section

A grant has been awarded to Dr. Paul Berry and Mr. Andrew Hipp at the University of Wisconsin-Madison to study the evolutionary history of part of the largest group of flowering plants in the North American continent, namely the sedge genus Carex. The investigators will use several levels of DNA analysis to determine relationships at both broad and fine scales in the genus. Broad scale DNA sequence studies will determine the relationships of several major groups within Carex, which is an important wetland plant throughout the U.S., Europe, China, and Japan. Fine scale molecular studies will use DNA fingerprinting tools called AFLP analysis to determine relationships between closely related species. Besides clarifying the relationships within one of the world's largest and ecologically important groups of flowering plants, this study will evaluate and develop new methods for analyzing plant molecular data using the amplified fragment length polymorphism (AFLP) technique. This is a sort of genetic fingerprinting tool, but the interpretation of plant AFLP results so far is less advanced than in animal DNA analysis for medical or criminal purposes. More specifically, this study aims to improve the techniques designed to determine the proper genealogy in closely knit groups of plants. As such, they could have broad applications for studies of rare and endangered species and conservation biology in general.

威斯康星大学麦迪逊分校的 Paul Berry 博士和 Andrew Hipp 先生获得了一笔资助，用于研究北美大陆最大的开花植物群体（即莎草属苔草）的部分进化历史。研究人员将使用多个级别的 DNA 分析来确定该属中广泛和精细尺度的关系。大规模的 DNA 序列研究将确定苔草内几个主要类群的关系，苔草是遍布美国、欧洲、中国和日本的重要湿地植物。精细规模的分子研究将使用称为 AFLP 分析的 DNA 指纹识别工具来确定密切相关的物种之间的关系。 除了阐明世界上最大且具有重要生态意义的开花植物群体之一之间的关系外，本研究还将评估和开发使用扩增片段长度多态性（AFLP）技术分析植物分子数据的新方法。这是一种基因指纹识别工具，但迄今为止对植物 AFLP 结果的解释不如用于医疗或犯罪目的的动物 DNA 分析先进。更具体地说，这项研究旨在改进旨在确定紧密结合的植物群体中正确谱系的技术。因此，它们可以在稀有和濒危物种以及保护生物学的研究中具有广泛的应用。