RUI: An HPLC-MS for Conducting Studies of Responses to Environmental Stresses in Marine Organisms

RUI：用于研究海洋生物对环境压力的反应的 HPLC-MS

• 批准号: 0300970
• 负责人: Kathryn Van Alstyne
• 金额: $ 16.47万
• 依托单位: Western Washington University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0300970&HistoricalAwards=false
• 关键词: RUI; HPLC; MS; Conducting; Studies

This award provides support for purchase of a high performance liquid chromatography - mass spectrometer (HPLC-MS) for the Shannon Point Marine Center (SPMC) of Western Washington University. Because of the Center's location, SPMC scientists have been actively involved in research on the local marine flora and fauna and in training students in marine research. In recent years, faculty research at the Center has become more focused on the physiology and biochemistry of marine organisms. Many of these studies involve the measurement of physiological and biochemical responses of organisms to changing environmental conditions. The use of HPLC is an important tool for such research because it allows researchers to identify, separate, and measure individual metabolites as their amounts change in response to a variety of environmental conditions. The scope of the studies that can be conducted at SMPC has been limited by a lack of appropriate mass spectroscopy capabilities throughout the entire Western Washington campus. The combination of HPLC and MS allows researchers to confirm identifications of known compounds and aid structural determination of newly discovered natural products. The instrument to be purchased, which has both analytical and preparative-scale capabilities, will be located in a new common-use analytical laboratory at the Center where it can be accessed by researchers at the Center and those from the main campus in Bellingham as well. Shannon Point has a strong record in supporting graduate and undergraduate research and education programs. The instrument will be available to students at all levels, including undergraduates participating in two NSF-supported training activities: the Minorities in Marine Science Undergraduate Program (MIMSUP) and the Research Experience for Undergraduates (REU) Site program.

该奖项为西华盛顿大学香农角海洋中心 (SPMC) 购买高性能液相色谱-质谱仪 (HPLC-MS) 提供支持。 由于该中心的地理位置，SPMC 的科学家一直积极参与当地海洋动植物群的研究以及对海洋研究学生的培训。近年来，中心教师的研究更加关注海洋生物的生理学和生物化学。 其中许多研究涉及测量生物体对不断变化的环境条件的生理和生化反应。 HPLC 的使用是此类研究的重要工具，因为它使研究人员能够识别、分离和测量单个代谢物，因为它们的量随各种环境条件而变化。 由于整个西华盛顿校区缺乏适当的质谱分析能力，SMPC 可以进行的研究范围受到限制。 HPLC 和 MS 的结合使研究人员能够确认已知化合物的鉴定并帮助确定新发现的天然产物的结构。所购买的仪器具有分析和制备规模功能，将位于该中心的一个新的通用分析实验室中，该中心的研究人员以及贝灵厄姆主校区的研究人员都可以使用该仪器。 Shannon Point 在支持研究生和本科生研究和教育项目方面拥有良好的记录。 该工具将可供各级学生使用，包括参加两项 NSF 支持的培训活动的本科生：海洋科学少数群体本科项目 (MIMSUP) 和本科生研究体验 (REU) 现场项目。