CAREER: Towards Synthetic Cytoplasm: Volume Exclusion and Aqueous Phase Separation in Giant Vesicles

职业：走向合成细胞质：巨囊泡中的体积排除和水相分离

• 批准号: 0239629
• 负责人: Christine Keating
• 金额: $ 51.2万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-02-15 至 2009-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0239629&HistoricalAwards=false
• 关键词: CAREER; Towards; Synthetic; Cytoplasm; Volume

This CAREER award to Professor Christine Keating of Pennsylvania State University is supported by the Analytical and Surface Chemistry Program in the Chemistry Division and by Cellular Systems in the Division of Molecular and Cellular Biosciences. In this project, the properties of aqueous two-phase systems will be studies as contained within giant unilamellar vesicles. Questions to be addressed include the partitioning of proteins and other macromolecules between the two phases, and how this partitioning is affected by encapsulation within the vesicle. The partitioning could either follow Michaelis-Menton or may show a sigmoidal, i.e., "switchlike" response curve, with the latter giving access to unique vesicle properties. One property to be examined is vesicle locomotion based on the reversible polymerization of cytoskeletal proteins within these "synthetic cells" with the driving force provided by a temperature and/or chemical gradient. The overall goal of the project is elucidation of the functional architectures used by cells, a knowledge which can then be applied to abiotic nanostructures.A comprehensive education and outreach plan is described, including outreach to high school students through a high school biology teacher. High school teachers have already worked in the PI's lab during summer months and a new teacher is to be supported for one summer with this award. These teachers have worked with state-of-the-art instrumentation and have been key in development of new experiments with biosensors and cell structure appropriate for the high school laboratory. In addition, the PI will develop a new graduate-level course on biorecognition.

宾夕法尼亚州立大学克里斯汀·基廷（Christine Keating）教授的职业奖得到了化学部门的分析和表面化学计划的支持，并由分子和细胞生物科学的分区中的蜂窝系统提供。 在该项目中，水溶液系统的性质将是巨型Unilamellar囊泡中包含的研究。 要解决的问题包括对两个阶段之间的蛋白质和其他大分子的分配，以及该分区如何受囊泡内包裹的影响。 分区可以遵循Michaelis-Menton，也可以显示出Sigmoidal，即“开关式”响应曲线，后者可以访问独特的囊泡特性。 要检查的一种特性是基于这些“合成细胞”中细胞骨架蛋白的可逆聚合的囊泡运动，并用温度和/或化学梯度提供的驱动力。 该项目的总体目标是阐明细胞使用的功能架构，然后将其应用于非生物纳米结构。描述了一项全面的教育和外展计划，包括通过高中生物学老师向高中生提供外展。 高中老师在夏季的几个月中已经在PI的实验室工作了，一个夏天的新老师将获得这个奖项。 这些老师已经使用了最先进的仪器，并且是针对高中实验室的生物传感器和细胞结构进行新实验的关键。 此外，PI将开发新的研究生级课程。