RUI: Gene-Specific Importin Alpha Functions in Development

RUI：开发中的基因特异性导入 Alpha 功能

• 批准号: 0234751
• 负责人: Robert Fleming
• 金额: $ 13.03万
• 依托单位: Trinity College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-01-15 至 2006-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0234751&HistoricalAwards=false
• 关键词: RUI; Gene; Specific; Importin; Alpha

0234751FlemingIn eukaryotic cells, cytoplasmic and nuclear compartments are separated by the nuclear envelope. This double membrane structure contains numerous nuclear pores that regulate the flow of molecular information into and out of the nucleus. Proper functioning of this transport process is essential for normal cellular metabolic regulation as well as the transmission of developmental signals from the extracellular and cytoplasmic compartments to the nucleus so that gene transcription can be regulated and modified during cellular differentiation processes.Many specific transporter molecules have been identified in this process. In particular, Dr. Fleming will focus on the importin a (imp a) genes act as adapter proteins for cargo molecules containing classical nuclear localization signals. The imp a gene family contains three distinct members (clades): imp a1, a2 and a3. Metazoan animals generally have representatives of each of the three clades. The evolutionary conservation of all three clades from Drosophila through human suggests that each clade is constrained in its ability to diverge from or converge on the others, suggesting that each clade has evolved specialized functions. He has chosen to examine the imp a gene family in Drosophila since there are only single genes representing each of the clades, thus allowing us to assess any specific functions of individual clade members.The merit of these studies resides in the definitive demonstration of unique roles for specific imp a genes in nuclear transport and perhaps roles for these genes in other cellular processes. The elucidation of these functions is critical for the understanding of cellular signaling and homeostasis. It is expected that these roles will be conserved in other metazoan animals having representative members of the different imp a clades. His studies will reveal evolutionary conservation of specific functions by comparisons with other species. Additionally, the experimentation within this proposal will provide undergraduate students with direct exposure to current biological methodologies while demonstrating the extent to which conclusions can be drawn from available data. This exposure will allow students to design additional experiments based on their findings and explore the true nature of scientific thought and experimentation. It is further anticipated that students will experience the opportunity to present their findings at local and national meetings and also in publications to experience the totality of the scientific community and the interactions fostered by it.

0234751荧光素真核细胞，细胞质和核室被核包膜分离。 这种双膜结构包含许多核孔，这些核孔调节分子信息进入和流出细胞核。 这种传输过程的正确功能对于正常的细胞代谢调节以及从细胞外和细胞质区室传播发育信号到细胞核至关重要。在此过程中已经确定了在细胞分化过程中可以调节和修改基因转录的基因转录。特别是，弗莱明博士将重点介绍含有经典核定位信号的货物分子的衔接蛋白（IMPA）基因。 IMP一个基因家族包含三个不同的成员（进化枝）：IMP A1，A2和A3。后生动物通常具有三个进化枝中的每个进化枝的代表。从果蝇到人类对所有三个进化枝的进化保护表明，每个进化枝都在其与其他进化枝脱离或收敛的能力上都受到限制，这表明每个进化枝都会进化出专门的功能。 他选择检查果蝇中的IMP基因家族，因为只有单个基因代表每个进化枝，从而使我们能够评估各个进化枝成员的任何特定功能。这些研究的优点存在于明确的核能中特定IMP A基因在核运输中的独特作用，也许是这些基因在其他细胞过程中的作用。这些功能的阐明对于理解细胞信号传导和稳态至关重要。 预计这些角色将在其他具有不同IMP a的代表成员的后生动物中保存。他的研究将通过与其他物种进行比较来揭示对特定功能的进化保护。此外，本提案中的实验将为本科生直接接触当前的生物学方法论，同时证明可以从可用数据中得出结论的程度。 这种接触将使学生根据他们的发现设计其他实验，并探索科学思想和实验的真实本质。 可以进一步预料到，学生将获得机会在当地和国家会议上以及出版物中介绍他们的发现，以体验整个科学界以及培养的互动。