Collaborative Research Proposal: Development of a Combined In Situ Particle Imaging Velocimeter / Fluorescence Imaging System

合作研究提案：开发组合式原位粒子成像测速仪/荧光成像系统

• 批准号: 0220213
• 负责人: Stephen Monismith
• 金额: $ 26.9万
• 依托单位: Stanford University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-15 至 2006-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0220213&HistoricalAwards=false
• 关键词: Collaborative; Research; Proposal; Development; Combined

The PIs propose to build a new system that will image multiple wavelengths of fluoresced and scattered light and perform stereo particle image velocimetry (3D PIV) measurements. These data will enable maps of fluid flow and derived quantities such as strain rate, vorticity, and the dissipation rate of turbulent kinetic energy to be produced. Measurements will be made on identical scales (20 x 20 x 0.6 cm with 100 um resolution), nearly simultaneously, allowing in situ 3D mapping of biological distributions to microscale physical flows. It is proposed that the multiple imaging wavelengths will allow a degree of taxonomic discrimination among fluorescent particles, while the side-scattered light will provide images of all particles that scatter the excitation irradiance including most of the phytoplankton appearing in the fluorescence images, as well as zooplankton. The 3D PIV-multispectral imaging fluorometer will be mounted on a free-falling platform with a suite of auxiliary instruments and a water sampler for image ground-truthing that has already been constructed and shown to provide undisturbed views of plankton in the water column. Images will be captured in a plane extending below the platform to obtain distributions unaffected by the platform. Shipboard epifluorescence microscopy will be used to identify dominant organisms in the images.Relating microscale distributions of plankton to microscale physical dynamics in the ocean has been largely limited by a lack of technology. The combination of multiple-wavelength imaging and PIV will allow in situ investigations of fluorescent particle/bulk fluorescence relationships, microscale distributions of plankton in relation to microscale flows, spatial relationships of grazers and primary producers, and physical mechanisms underlying fine- and microscale biological patchiness. This instrument is an enhancement of an existing 2D imaging fluorometer developed by the PIs that has only one sensing wavelength, and does not gather simultaneous physical data on the same scales as the fluorescence images.

PI提议构建一个新系统，该系统将对荧光和散射光的多个波长进行成像，并执行立体粒子图像速度测定（3D PIV）测量。这些数据将实现流体流量和衍生量（例如应变速率，涡度和要产生的湍流动能的耗散速率）的图。将几乎同时对相同的量表进行测量（20 x 20 x 0.6厘米，分辨率为100 UM），从而使生物分布的原位3D映射到显微镜物理流程。有人提出，多个成像波长将允许在荧光颗粒之间进行一定程度的分类歧视，而侧面散射光将提供所有分散激发辐射的颗粒的图像，包括大多数浮游植物出现在荧光图像中，以及浮游动物。 3D PIV-Multispectral Imaging荧光计将安装在带有一套辅助仪器和用于图像接地的水上采样器的自由落体平台上，这些辅助仪器已经构造并显示出可以在水柱中提供浮游生物的不受干扰的视图。图像将被捕获在平台下方延伸的平面上，以获取不受平台影响的分布。船上的落叶显微镜将用于识别图像中的主要生物。将浮游生物的显微镜分布与海洋中的显微镜物理动力学相关，这在很大程度上受到缺乏技术的限制。多波长成像和PIV的组合将允许对荧光颗粒/体积荧光关系，浮游生物的显微镜分布，与微观流相关的显微镜分布，放牧者和主要生产者的空间关系以及细菌生物学和微观生物学斑块的背后的物理机制。该仪器是PIS开发的现有2D成像荧光计的增强，该仪器仅具有一个感应波长，并且不会在与荧光图像相同的尺度上同时收集物理数据。