RUI: Genetic and Molecular Analysis of Photoperiod Flowering in Arabidopsis

RUI：拟南芥光周期开花的遗传和分子分析

• 批准号: 0215504
• 负责人: Karen Hicks
• 金额: $ 27万
• 依托单位: Kenyon College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-15 至 2006-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0215504&HistoricalAwards=false
• 关键词: RUI; Genetic; Molecular; Analysis; Photoperiod

0215504HicksEnvironmental factors such as light and temperature play a major role in regulating plant development and physiology, and have a significant impact on agricultural production. This proposal concerns the molecular and genetic basis for the regulation of flowering time by daylength, or photoperiod, focusing on the model plant Arabidopsis thaliana. Although recent progress has significantly increased the understanding of photoperiodic regulation of flowering, it is apparent that a number of key components of this signal transduction pathway are still unknown.Mutations at the EARLY FLOWERING 3 locus offer a unique inroad to the interactions between biological clocks and complex developmental processes such as floral initiation and light regulation. New genes that influence photoperiodic flowering will be identified and analyzed by isolating modifiers of the elf3 mutant phenotype using a genetic screen. Modifiers of the strong reduced function elf3-1 allele that restore photoperiod sensitivity (pse mutants) are likely to identify proteins that function downstream of ELF3 in the photoperiodic pathway. A number of mutations that restore photoperiod sensitivity in the absence of ELF3 function have already been identified. New loci identified in this modifier screen will be mapped using PCR-based molecular markers, and positional or candidate gene cloning will be undertaken for at least two modifier loci. In addition, this project aims to understand the links between photoperiodic regulation of flowering, photomorphogenesis, and circadian regulation. Phenotypic analysis of pse elf3-1 double mutants will test the hypothesis that these three processes are genetically separable.The discovery of new genes that influence photoperiodic flowering time will add to the repertoire of loci that are known to affect the transition to reproductive growth and might be manipulated to increase agricultural yield. An integral component of this project is the training and mentoring of undergraduate students. Their involvement in this research program will foster the development of laboratory skills and intellectual abilities that will enrich their lives and enable them to contribute to further progress in science.

0215504Hicks光、温度等环境因素对植物发育和生理起着主要调节作用，对农业生产产生重大影响。该提案涉及通过日长或光周期调节开花时间的分子和遗传基础，重点关注模式植物拟南芥。尽管最近的进展显着增加了对开花光周期调节的理解，但显然该信号转导途径的许多关键组成部分仍然未知。 EARLY FLOWERING 3 基因座的突变为生物钟和花期之间的相互作用提供了独特的途径。复杂的发育过程，例如花的起始和光调节。通过使用遗传筛选分离 elf3 突变体表型的修饰因子，将鉴定和分析影响光周期开花的新基因。恢复光周期敏感性的强功能减弱的 elf3-1 等位基因的修饰物（pse 突变体）可能会识别在光周期途径中 ELF3 下游发挥作用的蛋白质。已经鉴定出许多在 ELF3 功能缺失的情况下恢复光周期敏感性的突变。在此修饰筛选中鉴定的新基因座将使用基于 PCR 的分子标记进行定位，并且将对至少两个修饰基因座进行定位或候选基因克隆。此外，该项目旨在了解开花的光周期调节、光形态发生和昼夜节律调节之间的联系。 pse elf3-1 双突变体的表型分析将检验这三个过程在遗传上可分离的假设。影响光周期开花时间的新基因的发现将增加已知影响生殖生长过渡的基因座库，并可能进行操纵以提高农业产量。该项目的一个组成部分是本科生的培训和指导。他们参与这项研究计划将促进实验室技能和智力的发展，这将丰富他们的生活，并使他们能够为科学的进一步进步做出贡献。