U.S.-Czech Republic Research on Microarray-Based Analysis of Plant Genome Structure.

美国-捷克共和国基于微阵列的植物基因组结构分析研究。

• 批准号: 0130671
• 负责人: David Galbraith
• 金额: $ 4.04万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0130671&HistoricalAwards=false
• 关键词: U.S.; Czech; Republic; Research; Microarray

This U.S.-Czech research project between David Galbraith of the University of Arizona and his counterpart, Jaroslav Dolezel from the Czech Academy of Sciences' Institute of Experimental Botany in Olomouc, features use of flowsorting to selectively enrich populations of individual barley chromosomes. Their intent is to produce fluorescent genomic DNA targets for hybridization of barley DNA microarrays and for fluorescent in situ hybridization (FISH) analyses of metaphase chromosomes. The total effort benefits from the Arizona team's extensive expertise with microarray technologies and the Czech's expertise in both fluorescence-activated sorting of plant chromosomes and plant chromosome cytology. Research experiences for undergraduate students are an integral part of the research plan. If successful, this work my yield new techniques for mapping single copy genes (i.e. ESTs) on specific plant chromosomes using barley as a model. The researchers also anticipate using the sorted chromosomes for the production of chromosome-specific DNA libraries. This international project in plant genome research fulfills the program objective of advancing scientific knowledge by enabling experts in the Untied States and Central Europe to combine complementary talents and share research resources in areas of strong mutual interest and competence.

这个美国-捷克研究项目由亚利桑那大学的 David Galbraith 和捷克科学院奥洛穆茨实验植物研究所的 Jaroslav Dolezel 合作，其特点是使用流式分选来选择性富集单个大麦染色体的群体。他们的目的是生产荧光基因组 DNA 靶标，用于大麦 DNA 微阵列杂交和中期染色体荧光原位杂交 (FISH) 分析。整个工作得益于亚利桑那团队在微阵列技术方面的广泛专业知识以及捷克团队在植物染色体荧光激活分选和植物染色体细胞学方面的专业知识。本科生的研究经历是研究计划的一个组成部分。 如果成功，这项工作将产生新技术，以大麦为模型，在特定植物染色体上绘制单拷贝基因（即 EST）。研究人员还预计使用分选的染色体来生产染色体特异性 DNA 文库。这个植物基因组研究国际项目通过使美国和中欧的专家能够在共同感兴趣和能力强的领域结合互补人才并共享研究资源，实现了推进科学知识的计划目标。