Analysis of the Ethylene Signal-Transduction Pathway in Arabidopsis

拟南芥乙烯信号转导途径分析

• 批准号: 0131564
• 负责人: Brad Binder
• 金额: $ 42万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-15 至 2006-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0131564&HistoricalAwards=false
• 关键词: Analysis; Ethylene; Signal; Transduction; Pathway

The hormone ethylene regulates a number of processes in higher plants including fruit ripening, tissue aging, shedding of fruits and leaves, wound responses, and growth. This research is a continuation of previous efforts by this lab to investigate the mechanisms by which the Arabidopsis ETR family of ethylene receptors sense ethylene and trigger responses in the plant cells. Toward this end several important new tools have been or are being developed, including: 1) mutant plants that are deficient in each of the 5 receptor proteins; 2) a system for producing receptor proteins in yeast cells for biochemical analysis; and 3) a high resolution method for assessing rapid changes in seedling growth in response to ethylene. Using these basic systems, the mechanisms by which ethylene is perceived by the ETR receptors and how this binding is transmitted through the receptor protein to downstream effector proteins is being investigated. Effort is also being devoted to sorting out the specific roles that the different isoforms of the receptor play in the transduction of the ethylene signal. This research will lead to a better understanding of the mechanisms by which ethylene works and allow us to develop new, less toxic ways to prevent crop spoilage and control ripening processes.

乙烯激素调节高等植物的许多过程，包括果实成熟、组织老化、果实和叶子的脱落、伤口反应和生长。这项研究是该实验室之前研究拟南芥 ETR 家族乙烯受体感知乙烯并触发植物细胞反应的机制的延续。为此，已经或正在开发一些重要的新工具，包括：1）缺乏 5 种受体蛋白中每一种的突变植物； 2) 在酵母细胞中产生受体蛋白用于生化分析的系统； 3) 一种高分辨率方法，用于评估乙烯对幼苗生长的快速变化。使用这些基本系统，正在研究 ETR 受体感知乙烯的机制以及这种结合如何通过受体蛋白传递到下游效应蛋白。人们还致力于弄清楚受体的不同亚型在乙烯信号转导中所发挥的具体作用。这项研究将有助于更好地了解乙烯的作用机制，并使我们能够开发新的、毒性较小的方法来防止作物腐败和控制成熟过程。