Transformative Iron Metal Nanoparticles with Controlled Oxidation for Magnetic Particle Imaging.

用于磁粒子成像的具有受控氧化的变革性铁金属纳米粒子。

基本信息

批准号：
10730728
负责人：
JACQUELINE A JOHNSON
金额：
$ 45.11万
依托单位：
UNIVERSITY OF TENNESSEE SPACE INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-01 至 2025-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10730728
关键词：
4T1 Biodistribution Biology Cardiovascular system Cell Line Cells Characteristics Chemistry Contrast Media Development Dimensions Doctor of Philosophy Elements Environment Exposure to FDA approved Human Image In Vitro Internships Iron Label Ligands Macrophage Magnetic Resonance Imaging Magnetic nanoparticles Magnetism Mammalian Cell Mammary Neoplasms Measurement Metals Methods Molecular Mossbauer Spectroscopy Mus Optics Oxides Oxygen Performance Phase Polymers Property Research Research Assistant Research Project Grants Resistance Resolution Roentgen Rays Signal Transduction Specificity Structure Students Subcellular structure Techniques Thinness Time Tissues Toxic effect Training Transmission Electron Microscopy Work X ray diffraction analysis biological systems biomaterial compatibility contrast enhanced contrast imaging design ethylene glycol experience fabrication imaging modality improved in vivo insight instrument instrumentation iron oxide iron oxide nanoparticle nanoparticle novel oxidation particle physical property preservation summer research undergraduate student

4T1 Biodistribution Biology Cardiovascular system Cell Line Cells Characteristics Chemistry Contrast Media Development Dimensions Doctor of Philosophy Elements Environment Exposure to FDA approved Human Image In Vitro Internships Iron Label Ligands Macrophage Magnetic Resonance Imaging Magnetic nanoparticles Magnetism Mammalian Cell Mammary Neoplasms Measurement Metals Methods Molecular Mossbauer Spectroscopy Mus Optics Oxides Oxygen Performance Phase Polymers Property Research Research Assistant Research Project Grants Resistance Resolution Roentgen Rays Signal Transduction Specificity Structure Students Subcellular structure Techniques Thinness Time Tissues Toxic effect Training Transmission Electron Microscopy Work X ray diffraction analysis biological systems biomaterial compatibility contrast enhanced contrast imaging design ethylene glycol experience fabrication imaging modality improved in vivo insight instrument instrumentation iron oxide iron oxide nanoparticle nanoparticle novel oxidation particle physical property preservation summer research undergraduate student

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项目摘要

Project Summary This study focusses on the synthesis and characterization of an entirely new class of iron nanoparticles that transition to iron oxide nanoparticles (in vivo) over a controllable period consistent with providing biocompatibility and novel function. After synthesis, the particles will be characterized to reveal their size, structure, size distribution and magnetic properties. The degree of oxidation of FeNPs will be assessed by Mössbauer spectroscopy. The in vitro biocompatibility will be determined at various extents of oxidation. The magnitude of magnetic particle imaging signal and resolution will be determined as a function of FeNP oxidation. The Specific Aims of our research include: 1) The synthesis of iron nanoparticles for biomedical applications; 2) physical characterization and magnetic property determination of the iron nanoparticles; and 3) quantitative characterization of FeNPs, biocompatibility and MP image contrast capability in mammalian cells. We will create iron nanoparticles of uniform size with coatings that allow controllable oxidation. We will then demonstrate the transformative capabilities of these materials in producing a more sensitive nanoparticle for magnetic particle imaging. The new nanoparticle performance will be compared with VivoTrax, a commercial FeOxNP. Dr. Jacqueline Johnson’s group has performed preliminary research in the development of these nanoparticles and Dr. Johnson is a leading expert on the analysis of magnetic materials using Mössbauer spectroscopy as well as many preliminary characterization methods. Dr. Giorgio’s group has extensive experience in appropriate coatings of nanoparticles and transmission electron microscopy studies. Dr. Lu is an expert in nanoparticle chemistry; Dr. Lu and graduate research assistant, Aleia Williams, have been developing synthesis methods of FeNPs since 2020. This project will form the basis of Aleia’s and Vanderbilt GRA’s PhD work, summer research for interns at UTSI and research projects for resident undergraduates at Vanderbilt, for the duration of the project. In total, this is training for 8-10 students at various levels. The group will assess imaging performance as investigated by the company Magnetic Insight, who are developing instrumentation for Magnetic Particle Imaging.

项目摘要这项研究的重点是全新的铁的综合和表征在受控时期过渡到氧化铁纳米颗粒（体内）的纳米颗粒提供生物相容性和新功能的一致性。合成后，粒子将被表征以揭示其大小，结构，大小分布和磁性。 FENP的氧化程度将通过 Mössbauer光谱法。体外生物相容性将在各种范围内确定氧化。磁性粒子成像信号和分辨率的大小将是确定为FENP氧化的函数。我们研究的具体目的包括：1）纳米颗粒的合成生物医学应用； 2）物理表征和磁性确定铁纳米颗粒的； 3）FENP，生物相容性的定量表征哺乳动物细胞中的MP图像对比能力。我们将创建具有均匀尺寸的铁纳米颗粒，并具有允许控制的涂层氧化。然后，我们将在产生更灵敏的纳米颗粒用于磁颗粒成像。新的纳米颗粒性能将与商业feoxnp的Vivotrax进行比较。杰奎琳·约翰逊博士的小组在开发方面进行了初步研究这些纳米颗粒和约翰逊博士是磁分析的领先专家使用Mössbauer光谱以及许多初步表征的材料方法。乔治博士的小组在适当的涂料方面拥有丰富的经验纳米颗粒和透射电子显微镜研究。 Lu博士是专家纳米颗粒化学； Lu博士和研究生研究助理Aleia Williams一直是自2020年以来开发FENP的合成方法。该项目将构成 Aleia和Vanderbilt GRA的博士学位，UTSI实习生的夏季研究和研究在该项目期间，范德比尔特（Vanderbilt）的居民本科生的项目。总共这是对8-10名学生的培训。小组将评估想象力公司磁性见解的绩效，正在开发磁性颗粒成像的仪器。