PECASE: Development of a New Mass Spectrometry-Based Approach for Assaying Protein Stability and Initiation of a High School Science Teacher Outreach Program

PECASE：开发一种新的基于质谱的方法来测定蛋白质稳定性并启动高中科学教师外展计划

• 批准号: 0094224
• 负责人: Michael Fitzgerald
• 金额: $ 53万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-02-15 至 2006-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0094224&HistoricalAwards=false
• 关键词: PECASE; Development; New; Mass; Spectrometry

AbstractProposal: 0094224 Date: March 18, 2002PI: Michael C. Fitzgerald Institution: Duke University This PECASE award to Professor Michael Fitzgerald of Duke University is supported by the Analytical and Surface Chemistry Program in the Chemistry Division. The primary research objective is to develop a mass spectrometry based method to examine protein stability and folding. The experimental technique of hydrogen-deuterium exchange combined with matrix assisted laser desorption ionization mass spectrometry has several advantages over existing techniques for studying protein folding. The long-range goals of this research are to develop a method to study complex binding interactions between multiple proteins and DNA. The educational component of this program is both innovative and well developed. Dr. Fitzgerald presents a program focused on a summer workshop for high school science teachers. He collaborated with the school district science specialist in the development of the plan, which has also received the support of eight regional principals. This research will impact the fields of mass spectrometry and biochemistry. Compared to established methods, this method is not limited by sample purity and will have greater sensitivity. It is designed to be automated and fast. Future applications of this line of research could impact the study of both disease state and normal human health.

摘要Proposa：0094224日期：2002年3月18日：Michael C. Fitzgerald机构：杜克大学杜克大学迈克尔·菲茨杰拉德教授的Pecase奖。 主要的研究目标是开发一种基于质谱的方法来检查蛋白质稳定性和折叠。 氢 - 脱水氢交换的实验技术与基质辅助激光解吸电离质谱法相比，与研究蛋白质折叠的技术相比，具有多个优势。 这项研究的远程目标是开发一种研究多种蛋白质与DNA之间复杂结合相互作用的方法。 该计划的教育组成部分既具有创新性又发达。 菲茨杰拉德（Fitzgerald）博士提出了一项针对高中科学老师夏季研讨会的计划。 他与学区科学专家合作制定了该计划，该计划还得到了八名区域校长的支持。这项研究将影响质谱和生物化学领域。 与已建立的方法相比，此方法不受样品纯度的限制，并且具有更大的灵敏度。 它被设计为自动化和快速。这一研究的未来应用可能会影响疾病状态和正常人类健康的研究。