Molecular Level Analysis of Macromolecule-Surface Interactions in Bacterial Adhesion

细菌粘附中大分子-表面相互作用的分子水平分析

• 批准号: 0089156
• 负责人: Bruce Logan
• 金额: $ 255万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-15 至 2005-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0089156&HistoricalAwards=false
• 关键词: Molecular; Level; Analysis; Macromolecule; Surface

The Special Projects Office in the Chemistry Division supports this Collaborative Research Activities in Environmental Molecular Science (CRAEMS) Award to Penn State University. This award supports Bruce Logan, Darrel Velegol, Jon Chorover and James Kubicki to study macromolecule-surface interactions in bacterial adhesion in natural and engineered aquatic systems at the molecular level. In addition, the award supports Menachem Elimelech at Yale University through a subaward to study theoretical and experimental aspects of bacterial cell motion and adhesion near interfaces. The underlying hypothesis is that cell-surface interactions are dominated by the interaction of cell-surface polymers (exopolymers), and natural organic matter, such as humic and fulvic acids, with the surface. Atomic Force Spectroscopy, FTIR, electro-hydrodynamic techniques, and molecular modeling will be employed to probe polymer size, strength, and conformation as a function of solution chemistry, surface charge, and surface composition. The goal is improved methods and models that describe interactions between particles and surfaces that are irregularly shaped at atomic levels in the presence of bonded and soluble macromolecules.This research is designed to improve our understanding of bacterial adhesion in natural systems and in engineered environments. It has important implications for restoration of contaminated groundwater, water treatment, and wastewater reclamation, which will become increasingly important as availability of fresh water becomes a global environmental and human health concern. Outreach programs educate students of the local school system in environmental problems and approaches.

化学部的特别项目办公室支持宾夕法尼亚州立大学的环境分子科学合作研究活动（CRAEMS）奖。该奖项支持 Bruce Logan、Darrel Velegol、Jon Chorover 和 James Kubicki 在分子水平上研究自然和工程水生系统中细菌粘附的大分子-表面相互作用。此外，该奖项还通过一个子奖项支持耶鲁大学的 Menachem Elimelech 研究细菌细胞运动和界面附近粘附的理论和实验方面。基本假设是细胞表面相互作用主要由细胞表面聚合物（外聚物）以及天然有机物质（例如腐殖酸和黄腐酸）与表面的相互作用决定。原子力光谱、FTIR、电流体动力学技术和分子建模将用于探测聚合物尺寸、强度和构象作为溶液化学、表面电荷和表面组成的函数。目标是改进方法和模型，描述在键合和可溶性大分子存在的情况下，在原子水平上不规则形状的颗粒和表面之间的相互作用。这项研究旨在提高我们对自然系统和工程环境中细菌粘附的理解。它对受污染地下水的恢复、水处理和废水回收具有重要意义，随着淡水的供应成为全球环境和人类健康问题，这些将变得越来越重要。 外展计划对当地学校系统的学生进行环境问题和方法的教育。