Optical Biosensor for Marine Microbial Process Studies: Development Phase II

用于海洋微生物过程研究的光学生物传感器：开发第二阶段

• 批准号: 0083193
• 负责人: Paul Kemp
• 金额: $ 28.68万
• 依托单位: SUNY at Stony Brook
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-10-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0083193&HistoricalAwards=false
• 关键词: Optical; Biosensor; Marine; Microbial; Process

0083193KempThis technology development project will address how modern molecular techniques can provide new and fundamental insights into marine microbial communities and their activities. Studies of microbial processes have lagged far behind the technological revolution in oceanography. This project, applying biosensor technology, will continue development of a prototype fiber-optic, nucleic acid hybridization biosensor for rapid, quantitative detection of the abundance and expression of targeted bacterial genes, and the activity of targeted organisms in a mixed microbial community. The biosensor is novel in its design, in its application to marine microbial process studies, and in the proposed use of RNA targets that provide a natural signal amplification to increase sensitivity. The mail objective is to develop a highly sensitive, flexible instrument that can be applied to a broad suite of ecological problems. The components of the prototype system are relatively inexpensive, compact, and simple to operate. Procedures for extracting and purifying nucleic acids require ordinary laboratory skills and do not require any unusual equipment not already found in a typical molecular-biology laboratory. It is intended that the proposed instrument and analytical procedures be broadly useful and eventually be automated such that the system would be useful for remote/moored and continuous underway monitoring of marine microbial processes.

0083193Kemp 该技术开发项目将解决现代分子技术如何为海洋微生物群落及其活动提供新的基本见解。 对微生物过程的研究远远落后于海洋学的技术革命。 该项目应用生物传感器技术，将继续开发原型光纤核酸杂交生物传感器，用于快速定量检测目标细菌基因的丰度和表达，以及混合微生物群落中目标生物的活性。该生物传感器在设计、在海洋微生物过程研究中的应用以及提议使用提供自然信号放大以提高灵敏度的RNA靶标方面都是新颖的。邮件的目标是开发一种高度灵敏、灵活的工具，可应用于广泛的生态问题。 原型系统的组件相对便宜、紧凑且易于操作。提取和纯化核酸的程序需要普通的实验室技能，并且不需要典型分子生物学实验室中尚未发现的任何不寻常的设备。 其目的是使拟议的仪器和分析程序具有广泛的用途，并最终实现自动化，以便该系统可用于海洋微生物过程的远程/系泊和连续航行监测。