The Genetic and Molecular Basis of Sex Determination in Ceratopteris

角蕨性别决定的遗传和分子基础

• 批准号: 0077558
• 负责人: Jo Ann Banks
• 金额: $ 45万
• 依托单位: Purdue Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0077558&HistoricalAwards=false
• 关键词: Genetic; Molecular; Basis; Sex; Determination

AbstractGametophytes of the fern Ceratopteris richardii are sexually dimorphic, either hermaphroditic or male. The determinant of sex in this and other ferns is antheridiogen, a gibberellin-like pheromone that is secreted by the hermaphroditic gametophyte and promotes male development of other sexually undetermined gametophytes. The plant hormone abscisic acid blocks the antheridiogen response. The primary objective of this proposal is to understand how antheridiogen and abscisic acid govern the sex of the Ceratopteris gametophyte. The genetic analysis of five phenotypic classes of sex-determining mutants (12 loci) has led to a hypothetical sex-determining genetic pathway in this species. To understand how these genes regulate sex determination at the molecular level, it will be necessary to clone these genes. The objectives of the proposed research are to continue the genetic analysis of sex determination and develop methods required to clone the sex-determining genes in Ceratopteris. These methods include transformation, which will allow us to incorporate reverse genetic approaches to study gene function, and endogenous transposable element identification, which will provide new options to clone the many genes that have been identified by mutation. Understanding how sex is determined in plants is an important developmental question whose answer may ultimately have practical applications in agriculture and plant breeding. The proposed studies will provide a basic understanding of the genes involved in this process and will also be of value in understanding how the fate of a plant cell (the fern spore) is ultimately regulated by antheridiogen, a gibberellin-like pheromone.

蕨类植物菌的摘要摄氏菌是性二态性的，无论是雌雄同体还是男性。 在这种和其他蕨类植物中，性的决定因素是花药元，这是一种类似甘贝雷蛋白的信息素，由雌雄同体的配子植物分泌，并促进了其他性不确定的配子体的男性发展。 植物激素脱甲酸阻止了药力基因原子的反应。 该提议的主要目的是了解药力植物原和脱落酸如何控制ceratopteris配子体的性别。 对性别确定的突变体（12个基因座）的五类表型类别的遗传分析导致了该物种的假设性别确定的遗传途径。 为了了解这些基因如何在分子水平调节性别确定，需要克隆这些基因。 拟议的研究的目标是继续对性别确定的遗传分析，并开发克隆Ceratopteris中性别确定基因所需的方法。 这些方法包括转化，这将使我们能够结合研究基因功能的反向遗传方法和内源性转座元件鉴定，这将为克隆多种通过突变鉴定的基因提供新的选择。了解植物中的性别是如何确定的一个重要的发展问题，其答案最终可能在农业和植物育种中使用实际应用。 拟议的研究将提供对此过程中涉及的基因的基本理解，并且在理解植物细胞（蕨孢子）的命运最终如何受花药原（gibberellin样的信息素）调节方面也具有价值。