The Molecular Organization of Chromosome I: Control of Meiotic Recombination

I 号染色体的分子组织：减数分裂重组的控制

• 批准号: 9986096
• 负责人: David Kaback
• 金额: $ 12.5万
• 依托单位: Rutgers, The State University of New Jersey-RBHS-New Jersey Med
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2002-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9986096&HistoricalAwards=false
• 关键词: Molecular; Organization; Chromosome; Control; Meiotic

The molecular mechanism of interference (the reduction of the rate of crossing over between some chromosomes) is not known. It has been suggested that the meiotic synaptonemal complex (SC) is involved in mediating interference. Therefore, a better understanding of the mechanism of interference might be gained by investigating the yeast S. cerevisiae SC. This project will examine the dynamics of SC formation in wild type and ndt80 mutant cells and identify the proteins and genes encoding these proteins. SC will be labeled using a functional, fluorescent ZIP1-GFP hybrid protein constructed in the principal investigator's laboratory. The structure and dynamics of fluorescent SC will be examined using optical sections and fluorescent cinema-microscopy. Fluorescent SC will be purified and proteins contained in these structures extracted and identified by tandem mass spectroscopy. Genes corresponding to each gene product will be identified and ultimately mutated so their role in SC formation, crossover interference and other aspects of meiotic recombination and pairing can be tested.

干扰分子机制（在某些染色体之间的交叉速率降低）尚不清楚。 有人提出，减数分裂的突触复合物（SC）参与介导干扰。 因此，可以通过研究酵母菌S. SC来更好地理解干扰机理。该项目将检查野生型和NDT80突变细胞中SC形成的动力学，并鉴定编码这些蛋白质的蛋白质和基因。 SC将使用主要研究者实验室中构建的功能性的荧光Zip1-GFP杂交蛋白进行标记。 荧光SC的结构和动力学将使用光学切片和荧光电影 - 微观镜检查检查。 荧光SC将被纯化，并在这些结构中包含蛋白质，并通过串联质谱进行提取和鉴定。 与每个基因产物相对应的基因将被鉴定并最终突变，因此可以测试它们在SC形成，交叉干扰和减数分裂重组和配对的其他方面的作用。