CAREER: Synthesis, Characterization and Application of Fluorescent Nanolipobeads for Optochemical Sensing in Single Living Cells

职业：用于单活细胞光化学传感的荧光纳米脂珠的合成、表征和应用

基本信息

批准号：
9874498
负责人：
Zeev Rosenzweig
金额：
$ 31.7万
依托单位：
University of New Orleans
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
1999
资助国家：
美国
起止时间：
1999-07-01 至 2003-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9874498&HistoricalAwards=false
关键词：
CAREER Synthesis Characterization Application Fluorescent

项目摘要

This project of Professor Z. Rosenzweig of the University of New Orleans, supported by the Analytical and Surface Chemistry program, is a study of a new fluorescence based analytical method for determination of pH, and the levels of molecular oxygen and hydrogen peroxide in single living cells. The new method is less invasive and more rapid than methods such as fluorescence microscopy or cell cytometry, and is proposed to be less technically involved for the user. The measurements are indicative of exposure of the cell to oxidizing agents that are shown to be detrimental to the cell via formation of reactive oxygen species within the cell. The measurement is made by monitoring the fluorescent emission of nanolipobeads that are incorporated in the cell membranes. The educational aspects of the CAREER program are addressed via proposed revisions to the laboratory portion of the freshman chemistry laboratory and the incorporation of clinical chemistry experiments into an analytical chemistry graduate course.Individual cells are considered to be accurate and sensitive indicators of biological stress induced by environmental pollutants. The project of Professor Rosenzweig develops very small fluorescent beads that are incorporated into the cell membrane and provide a response that is an indicator of levels of reactive oxygen species inside the cell that are produced as a result of stress.

在分析和表面化学计划的支持下，新奥尔良大学的Z. Rosenzweig教授的这个项目是对基于荧光的新分析方法的研究，用于确定pH值，以及单个活细胞中的分子氧和过氧化氢的水平。新方法比荧光显微镜或细胞细胞术等方法较不侵入性，并且更快，并且建议用户在技术上涉及的涉及较少。测量结果表明细胞暴露于氧化剂中，这些氧化剂通过形成细胞内的活性氧对细胞有害。测量是通过监测纳米脂肪的荧光发射来进行的，这些纳米脂蛋白掺入了细胞膜中。通过对新生化学实验室的实验室部分进行修订，将职业计划的教育方面解决，并将临床化学实验纳入分析化学研究生课程中。个人细胞被认为是环境污染物引起的生物应激指标的准确和敏感指标。 Rosenzweig教授的项目会形成很小的荧光珠，这些荧光珠被掺入细胞膜中，并提供了一种响应，该响应是由于压力而产生的细胞内部活性氧水平的指标。