Activating Biocatalysts for Nonaqueous and Combinatorial Reactions

活化非水反应和组合反应的生物催化剂

• 批准号: 9811352
• 负责人: Jonathan Dordick
• 金额: $ 26.86万
• 依托单位: Rensselaer Polytechnic Institute
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-15 至 2002-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9811352&HistoricalAwards=false
• 关键词: Activating; Biocatalysts; Nonaqueous; Combinatorial; Reactions

This proposal pursues an older observation that the lyophilization of certain enzyme catalysts in the presence of highly ionic, non-buffering salts, e.g., KCl, can increase the catalytic rate in non-aqueous systems by 3-4 orders of magnitude. This has been demonstrated primarily on subtilisin. The specific aims of the original proposal are: To elucidate the mechanism(s) of enzyme activation in organic solvents with saltsTo extend the concept to a broad range of enzymes and reactions. To utilize the activated enzymes for combinatorial biocatalysis. In the revised and funded proposal, Aim 3 has been dropped and a more detailed examination of the mechanism and scope of the phenomenon has been added.

该提议追求了一个较旧的观察结果，即在存在高离子，非缓冲盐（例如KCl）存在下某些酶催化剂的冻干可以使非水系统中的催化速率提高3-4个幅度。这主要在枯草菌素上证明。原始提案的具体目的是：阐明具有盐的有机溶剂中酶激活的机制，将概念扩展到了广泛的酶和反应。 利用活化的酶进行联合生物催化。 在修订和资助的提案中，AIM 3已被删除，并且对现象的机制和范围进行了更详细的检查。