POWRE: Structure and Function of an Apoptosis Domain in the 75 kDa Neurotropin Receptor

POWRE：75 kDa Neurotropin 受体中凋亡结构域的结构和功能

• 批准号: 9805771
• 负责人: Barbara Chapman
• 金额: $ 7.5万
• 依托单位: California State University San Marcos Corporation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9805771&HistoricalAwards=false
• 关键词: POWRE; Structure; Function; Apoptosis; Domain

9805771 CHAPMAN During development, different tissues and organs require different numbers of cells. Programmed cell death (or "apoptosis"), along with cell division, is a primary way that cell number is controlled. Apoptosis occurs as a series of cellular and biochemical events in which the cell, instead of dividing, involutes, falls apart, and is engulfed. Every normal cell appears to have the "program" for cell death, which can be activated in a number of ways. The neurotrophin receptor gp75NTR or NTR, expressed by neurons and some immune cells, is a member of a family of cytokine and growth factor receptors known to regulate many cellular decisions to undergo programmed cell death. The NTR contains an intracellular domain that is similar to the "death domain" of other receptors that can initiate apoptosis, but it has not yet been shown that the NTR's putative death domain functions in apoptotic signaling. The objective of Dr. Chapman's study are to establish whether the NTR's death domain interacts with elements of the apoptotic mechanism (caspases), and to demonstrate whether this receptor can induce programmed cell death when expressed ectopically in cultured mammalian cells. This project is particular suitable for a POWRE award. Dr. Chapman recently made the unusual transition from the biotechnology industry to take a faculty job at the new San Marcos campus of the California State University system. CSUSM emphasized hands-on laboratory experience with modern technology to prepare their students in the biological sciences for careers as researchers, science teachers and in the biotechnology industry. Dr. Chapman brings real world experience to her students, and will be able to actively engage them in research with this POWRE award, which will provide the funding for her to initiate this research project.

9805771 CHAPMAN 在发育过程中，不同的组织和器官需要不同数量的细胞。 程序性细胞死亡（或“细胞凋亡”）以及细胞分裂是控制细胞数量的主要方式。 细胞凋亡作为一系列细胞和生化事件发生，其中细胞不是分裂，而是退化、分裂并被吞噬。 每个正常细胞似乎都有细胞死亡的“程序”，可以通过多种方式激活。 神经营养蛋白受体 gp75NTR 或 NTR，由神经元和一些免疫细胞表达，是细胞因子和生长因子受体家族的成员，已知可调节许多细胞决策以进行程序性细胞死亡。 NTR含有一个与其他受体的“死亡结构域”相似的胞内结构域，可以启动细胞凋亡，但尚未表明NTR的推定死亡结构域在细胞凋亡信号传导中发挥作用。 Chapman 博士研究的目的是确定 NTR 的死亡结构域是否与细胞凋亡机制的元件（半胱天冬酶）相互作用，并证明该受体在培养的哺乳动物细胞中异位表达时是否可以诱导程序性细胞死亡。 该项目特别适合获得 POWRE 奖。 查普曼博士最近从生物技术行业进行了不同寻常的转变，在加州州立大学系统的新圣马科斯校区担任教职。 CSUSM 强调现代技术的实验室实践经验，为生物科学领域的学生成为研究人员、科学教师和生物技术行业的职业做好准备。 Chapman 博士为她的学生带来了现实世界的经验，并将能够利用这个 POWRE 奖积极让他们参与研究，这将为她启动这个研究项目提供资金。