Glycosylation of Recombinant Proteins Research Planning Grant

重组蛋白糖基化研究计划资助

• 批准号: 9711130
• 负责人: Sarah Harcum
• 金额: $ 2.8万
• 依托单位: New Mexico State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-01 至 1998-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9711130&HistoricalAwards=false
• 关键词: Glycosylation; Recombinant; Proteins; Research; Planning

9711130 Harcum This Research Planning Grant proposal addresses what appears to be a commercially significant and scientifically under- investigated area, the effect of bioreactor conditions on the glycosylation patterns of protein. The research program would study mammalian cells grown in continuous culture (chemosts) and determine the effect of variables such as agitation, pH, temperature, growth rates, etc. Electrophoretic and chromatographic separations coupled with mass spec and NMR analyses will be used in structural determinations. ***

9711130 Harcum 这项研究规划拨款提案解决了一个看似具有商业意义且科学上尚未充分研究的领域，即生物反应器条件对蛋白质糖基化模式的影响。 该研究计划将研究在连续培养（化学）中生长的哺乳动物细胞，并确定搅拌、pH、温度、生长速率等变量的影响。电泳和色谱分离以及质谱和核磁共振分析将用于结构测定。 ***