SGER: Development of Ion Channels in Mouse Ventricular Zone Cells

SGER：小鼠心室区细胞离子通道的发育

• 批准号: 9708656
• 负责人: William Moody
• 金额: $ 4.22万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9708656&HistoricalAwards=false
• 关键词: SGER; Development; Ion; Channels; Mouse

IBN-9708656 PI: MOODY The neurons that populate the mammalian brain are generated during embryonic development from cells that line the ventricles. These cells divide repeatedly, and then migrate away from the ventricles to populate the brain itself. It is critically important that the processes of division and migration occur correctly in order for the brain to develop normally. Although it is well known that mature neurons in the brain use electrical signalling to process information, there is also some evidence that modified forms of electrical signalling are used during the processes of division and migration that create the brain during early embryogenesis. Dr. Moody will use quantitative techniques to record electrical signals from individual cells lining the ventricles of the embryonic mouse brain to determine how such signals are used in early brain development. By using living slices of embryonic brain tissue, it will be possible to identify individual cells according to their state of division and migration, and to then detemine the exact nature of the electrical signals they can generate. Once this is understood, specific drugs that block different aspects of those signals will be used to determine whether normal patterns of division and migration require this type of signalling. Results from this project will allow us to understand whether the normal adult forms of electrical communication between neurons in the brain are also used in modified form to mediate critical aspects of neural development.

IBN-9708656 PI：喜怒无常的神经元在胚胎发育过程中从脑室的细胞中产生了哺乳动物大脑的神经元。 这些细胞反复分裂，然后从心室迁移以填充大脑本身。 为了使大脑正常发展，分裂和迁移的过程非常重要。 尽管众所周知，大脑中的成熟神经元将电信号传导用于处理信息，但也有一些证据表明，在分裂和迁移过程中使用了修改的电信号传导形式，在早期胚胎发生过程中会产生大脑。 穆迪博士将使用定量技术记录来自胚胎小鼠大脑心室的单个细胞的电信号，以确定如何在早期大脑发育中使用此类信号。 通过使用胚胎脑组织的活片，可以根据分裂和迁移的状态识别单个细胞，然后忽略它们可以产生的电信号的确切性质。 一旦理解了这一点，将使用阻止这些信号不同方面的特定药物来确定划分和迁移的正常模式是否需要这种信号传导。 该项目的结果将使我们能够了解大脑中神经元之间的正常成人形式的电通通信形式是否也用于修改形式，以介导神经发育的关键方面。