Development of Electrical Properties in Neural-Lineage Cellsof Early Xenopus Embryos

早期非洲爪蟾胚胎神经谱系细胞电特性的发展

• 批准号: 8910254
• 负责人: William Moody
• 金额: $ 20.02万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1993-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8910254&HistoricalAwards=false
• 关键词: Development; Electrical; Properties; Neural; Lineage

This project will examine the development of electrophysiological properties in neural-lineage cells of the early Xenopus embryo. Although much attention has focussed on the modulation of populations of ion channels during the terminal differentiation of specific sets of post-mitotic neurons, very little information is available about the development of ion channel populations in neural-lineage cells at earlier stages, when initial commitment to neural phenotypes occurs. Dr. Moody will use patch clamp techniques to study this problem in the Xenopus embryo, at stages between the unfertilized oocyte and the neurula. He will examine the stages at which cells involved in the formation of the embryonic nervous system first express functional ion channel populations distinct from cells of other fates, and then investigate the developmental processes by which the electrical properties of the oocyte are transformed into those of cells committed to neuronal phenotypes. Particular attention will be paid to the roles of ion channel synthesis, the modulation of pre-existing channels, and the spatial distribution of ion channels over the surface of single cells in the early electrophysiological differentiation of the nervous system.

该项目将研究早期爪蟾胚胎神经锻炼细胞中电生理特性的发展。 尽管在特定的有丝分裂后神经元的终末分化过程中，当最初对神经表型的最初承诺发生时，很少有关于特定的有丝分裂后神经元的最终分化的离子通道种群的调节，但几乎没有信息的信息。 穆迪博士将使用斑块夹技术在无毛的卵母细胞和神经拉拉之间的各个阶段中研究这一问题。 他将检查与其他命运细胞不同的胚胎神经系统形成的细胞首次表达功能性离子通道种群，然后研究卵母细胞的电特性转化为致力于神经元表型的细胞的发育过程。 特别关注离子通道合成的作用，预先存在通道的调节以及在神经系统的早期电生理分化中，离子通道在单个细胞表面上的空间分布。