CAREER: New Methods of Creating Artifical Receptors for Proteins

职业：创建蛋白质人工受体的新方法

• 批准号: 9701975
• 负责人: Sanku Mallik
• 金额: $ 8万
• 依托单位: University of North Dakota Main Campus
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 1998-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9701975&HistoricalAwards=false
• 关键词: CAREER; New; Methods; Creating; Artifical

Professor Mallik's research focuses on the design, synthesis and evaluation of liposome based receptors for protein surfaces. Mixed liposomes derived from metal containing lipids, polymerizable ionic lipids, and polymerizable hydrogen bonding lipids are equilibrated with the template protein carbonic anhydrase to create a three-dimensional, complementary replica of the protein surface. Synthetic targets include lipids containing differing head groups and photopolymerizable diene or diyne tails that, after equilibration, are photochemically polymerized to achieve a semi-rigid, protein-specific receptor. Efficacy of the photopolymerized template synthesis is quantified by measuring the protein-replica binding constants. Educational activities include introducing a graduate bio-organic course and addressing undergraduate needs by establishing a Virtual Organic Chemistry classroom on the Web to facilitate student-teacher communication. With this CAREER award, the Organic Dynamics Program supports the research and educational activities of Professor Sanku Mallik of the Department of Chemistry at the University of North Dakota. Professor Mallik's research is focused on the creation of a three-dimensional protein recognition surface by using a specific protein to direct the formation of a collection of molecules with which it interacts. Subsequent chemical linking of these molecules into a polymer effectively leaves a footprint of the protein in the resulting polymer. In contrast to the considerable synthetic effort involved in other approaches to molecular recognition, this approach is expected to generate simply and rapidly a highly specific binding site for the target protein. Professor Mallik's educational activities include developing a graduate curriculum targeting the interface between organic and biological chemistry and facilitating student-teacher communication through a Virtual Classroom on the Web.

Mallik教授的研究重点是基于脂质体受体的蛋白质表面的设计，合成和评估。 用含脂质，可聚合的离子脂质和可聚合氢键的脂质衍生的混合脂质体与模板蛋白碳酸肺养酶平衡，以产生蛋白质表面的三维互补复制品。 合成靶标包括含有不同头部组的脂质和可光聚合的二烯或Diyne尾巴，在平衡之后，在光化学上聚集以获得半刚性的蛋白质特异性受体。通过测量蛋白质复合结合常数来量化光聚合模板合成的功效。 教育活动包括引入研究生生物有机课程，并通过在网络上建立虚拟有机化学课堂来满足本科需求，以促进学生教师的交流。 有了这个职业奖，有机动力学计划支持北达科他大学化学系Sanku Mallik教授的研究和教育活动。 Mallik教授的研究集中在创建三维蛋白识别表面，通过使用特定的蛋白质来指导与之相互作用的分子的形成。 随后将这些分子与聚合物的化学连接有效地使所得聚合物中的蛋白质占用。 与其他分子识别方法所涉及的相当大的合成工作相反，这种方法有望简单迅速地生成靶蛋白的高度特异性结合位点。 马利克教授的教育活动包括开发针对有机和生物化学之间界面的研究生课程，并通过网络上的虚拟教室来促进学生教师的交流。