MRI-R2: Acquisition of an automatic isothermal titration microcalorimeter

MRI-R2：购买自动等温滴定微量热计

• 批准号: 0959422
• 负责人: Sanku Mallik
• 金额: $ 35.18万
• 依托单位: North Dakota State University Fargo
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-15 至 2013-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0959422&HistoricalAwards=false
• 关键词: MRI; R2; Acquisition; automatic; isothermal

0959422Mallik"This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5)."This collaborative proposal from the North Dakota State University and the University of South Dakota requests funds to purchase an automatic isothermal titration microcalorimeter (auto-ITC200 from Microcal/GE Healthcare Biosciences) for Biomaterials research and education. The proposed instrument determines binding parameters (i.e., stoichiometry, association constant, enthalpy and entropy) in a single experiment, employing a very small sample size (5-10 g per experiment). The instrument determines binding constants in the range 102 - 109 M - 1 and the limit can be further enhanced by employing competitive binding techniques. It is fully automated to run 50 samples per day. Currently, 10 faculty researchers (25 graduate and 20 undergraduate students) at NDSU and USD are studying the interactions of peptide nanofibers, lipid- and polymer-based nanoparticles with proteins, DNA fragments, aptamers and antibodies. Complete thermodynamic characterization of the binding is a critical need for all of these research projects. However, the only ITC available is located in the Biochemistry Department at NDSU and the instrument is more than 12 years old (VP ITC from MicroCal Inc., purchased in 1997) and the injection assembly is not communicating properly with the control computer. The instrument is inadequate to satisfy the needs of the growing numbers of Biomaterials researchers of the two universities. In addition, this instrument is not set-up as a cost-center and often the access is fairly restricted. The proposed auto-ITC200 instrument will ensure that the faculty researchers and their students at NDSU and USD have access to a state-of-the-art microcalorimeter. This will also impart appropriate training to the graduate and the undergraduate students at both universities. The proposed instrument will be set-up as a cost-center and the generated user fees will be used for maintenance and upgrade. To effectively share the instrument between NDSU and USD, cyber infrastructure-based protocol will be followed for time allocation, data processing and data retrieval using the North Dakota NSF EPSCoR supported, high-speed network connection.

0959422-Mallik“该奖项是根据2009年的《美国复苏与再投资法》（公共法律111-5）资助的。所提出的仪器在单个实验中确定结合参数（即化学计量，结合常数，焓和熵），采用了很小的样本量（每个实验5-10 g）。该仪器确定了在102-109 m -1范围内的结合常数，并且可以使用竞争性结合技术进一步增强极限。它是完全自动化的，每天运行50个样品。目前，NDSU和USD的10位教职员工研究人员（25名毕业生和20名本科生）正在研究肽纳米纤维，脂质和聚合物基于脂质的纳米颗粒与蛋白质，DNA碎片，适应性和抗体的相互作用。对结合的完全热力学表征对所有这些研究项目都是至关重要的需求。但是，唯一可用的ITC位于NDSU的生物化学部门，该仪器已有12年以上的历史（从1997年购买的Microcal Inc.的VP ITC ITC），并且注射程序与控制计算机未正确通信。该工具不足以满足两所大学的生物材料研究人员的需求。此外，该仪器不是以成本为中心的设置，并且访问通常受到相当限制。拟议的Auto-ITC200仪器将确保NDSU和USD的教职员工研究人员及其学生可以使用最先进的微透明度计。这也将为两所大学的研究生和本科生提供适当的培训。所提出的仪器将设置为成本中心，生成的用户费用将用于维护和升级。为了有效地共享NDSU和USD之间的仪器，将遵循基于网络基础架构的协议，以使用北达科他州NSF EPSCOR支持的高速网络连接，以进行时间分配，数据处理和数据检索。