Genetic Modulation of Self-recognition in Brassica

芸苔属自我识别的基因调控

• 批准号: 9631921
• 负责人: Mikhail Nasrallah
• 金额: --
• 依托单位: Cornell Univ - State: AWDS MADE PRIOR MAY 2010
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-07-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9631921&HistoricalAwards=false
• 关键词: Genetic; Modulation; Self; recognition; Brassica

9631921 Nasrallah Genetic self-incompatibility, whereby the stigma of the flower is able to inhibit pollen germination and pollen tube development after self-pollination, represents one of the more promising systems for analyzing cell-cell communication in higher plants. In Brassica, Dr. Nasrallah has demonstrated that the self-incompatibility S locus is a multifunctional gene complex that controls signal perception through genes that are expressed in cells of the stigma epidermis and that encode plasma membrane- and cell wall-localized receptors. Thus, elucidation of the mechanism of self-incompatibility in this system is likely to provide insight into receptor-mediated cell-cell signaling in plants. They will continue their analysis of mutations that lead to the breakdown of self-incompatibility with the goal of identifying genes, either at the S locus or at unlinked loci, that encode the various components of the recognition and signal transduction phases of the self-incompatibility response. They will focus on a series of deletions that they have recently generated in strains carrying defined S haplotypes. Two classes of lesions that perturb the self-incompatibility response in stigma and/or pollen have been identified. In one class, the lesions map to the S locus and will be exploited to isolate novel genes required for the perception phase of the response. A second class of lesions results in the stigma-specific breakdown of self-incompatibility; these lesions affect a locus unlinked to the S locus which is a condidate for a gene controlling a step in the self-incompatibility signaling cascade. The study promises to influence our thinking on how plant cells transduce signals across their outer cell wall, how they utilize a precise molecular recognition system, and how a multi-component signal perception system has evolved and is maintained in a coadaped gene complex.

9631921 Nasrallah遗传自我不相容性，因此，花的污名能够抑制花粉发芽和自相传制后的花粉管的发育，这是分析高等植物中细胞细胞通信的更有前途的系统之一。在Brassica，Nasrallah博士证明了自我兼容性S位点是一种多功能基因复合物，可以通过在柱头表皮细胞中表达的基因来控制信号感知，并编码质膜和细胞壁钙化受体。因此，在该系统中阐明自我兼容的机制可能会洞悉植物中受体介导的细胞信号传导。他们将继续对突变的分析，从而导致自我兼容性的分解，以识别S基因座或未链接基因座的基因，这些突变的目标是编码自我兼容响应的识别和信号转导阶段的各种组成部分。他们将专注于最近在携带定义的S单体型的菌株中产生的一系列删除。已经确定了两类的病变，这些病变已经确定了污名和/或花粉中的自我兼容性反应。在一个类别中，病变映射到S基因座，并将被利用以分离响应感知阶段所需的新基因。第二类病变导致自我不相容性的特定污名分解。这些病变会影响与S基因座未链接的基因座，这是控制自我兼容信号级联步骤的基因的凝聚。该研究有望影响我们对植物细胞如何在其外部细胞壁上传导信号，如何利用精确的分子识别系统以及多组分信号感知系统如何发展并保持在共腺基因复合物中的思维。