NASA/NSF Collaborative Research: Calcium-Mediated Mechano- Sensory Signal Transduction in Plants

NASA/NSF 合作研究：植物中钙介导的机械感觉信号转导

基本信息

批准号：
9416038
负责人：
Jeffrey Harper
金额：
$ 54万
依托单位：
The Scripps Research Institute
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1994
资助国家：
美国
起止时间：
1994-09-01 至 2000-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9416038&HistoricalAwards=false
关键词：
NASA NSF Collaborative Research Calcium

项目摘要

9416038 Harper This research is focused on exploring the role of Calcium-Dependent Protein Kinases (CDPKs) in mechano-sensory signal transduction. The three specific aims are to: 1) Identify substrates for CDPK- dependent signal transduction pathway, 2) Test the hypothesis that the activity of CDPK isoform AK1 is critical to a mechano-sensory response, and 3) Develop a fluorescent tagging system to monitor dynamic changes in the membrane/cytoskeletal systems of live cells in response to physical stimulation. Since many mechano-sensory responses appear to involve calcium signals, it is important to determine what role CDPKs are playing in these phenomena. Using a CDPK signal transduction pathway as a focus, this research emphasizes the development of unique collaborations and new tools with far reaching implications for research in the field of plant cell biology. For example, the specific aim to identify CDPK substrates will involve a collaboration of researchers from all areas of plant biology. In the second specific aim, the transgenic plants expressing a mutant CDPK will be made available as a tool to all researchers seeking to define the signal transduction pathways involved in a plant's response to an environmental signal. Finally, it is hoped that the tool developed in the third specific aim, GFP tagged membrane/cytoskeletal systems, will provide a foundation for a powerful new approach in investigating the dynamics of cell structure. ***

9416038 HARPER这项研究的重点是探索钙依赖性蛋白激酶（CDPK）在机械感觉信号转导中的作用。这三个具体目的是：1）确定依赖CDPK的信号转导途径的底物，2）检验以下假设：CDPK同工型AK1的活性对于机械感应响应至关重要，并且3）开发荧光标记系统以监测膜/细胞骨架系统中活性细胞的动态变化。由于许多机械感知响应似乎涉及钙信号，因此确定CDPK在这些现象中扮演什么作用很重要。这项研究以CDPK信号转导途径为重点，强调了独特的合作和新工具的开发，对植物细胞生物学领域的研究产生了巨大影响。例如，识别CDPK底物的具体目的将涉及植物生物学各个领域的研究人员的合作。在第二个特定目的中，将向所有寻求定义植物对环境信号反应的信号转导途径的研究人员提供表达突变CDPK的转基因植物作为工具。最后，希望该工具在第三个特定目标（GFP标记的膜/细胞骨架系统）中开发的工具将为研究细胞结构动力学的强大新方法提供基础。 ***