National High Field FT-ICR Mass Spectrometry Facility

国家高场FT-ICR质谱装置

• 批准号: 9413008
• 负责人: Alan Marshall
• 金额: $ 500万
• 依托单位: Florida State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-01 至 1999-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9413008&HistoricalAwards=false
• 关键词: National; Field; FT; ICR; Mass

ABSTRACT PROPOSAL: 9413008 PI: MARSHALL, A. G. INSTITUTION: FLORIDA STATE UNIVERSITY This award from the Chemistry Research Instrumentation and Facilities Program will aid in the development of FT-ICR-Mass Spectrometry Facility in the National High Magnetic Field Laboratory at Florida State University. The research to be performed with this instrumentation include the following: direct sequencing, chemical reactivity, and shape analysis of gas-phase proteins up to antibody size (150,000 Da); direct optical absorption spectroscopy and electron paramagnetic resonance of mass-selected trapped ions; and routine direct identification of unseparated components of mixtures as complex as crude oil. This Facility will have the highest-field ICR mass spectrometers in the world. The technological development in FT-ICR will have major impact in biological and analytical chemistry, and the NSF center will enhance the spread of these development to a broad community of users. Methods Development at the facility will include research on: a) internal and external ionization techniques, b) ion Sources for thermolabile and/or solid compounds, c) IR laser desorption (LD) and d) vaporization of high boiling point mixtures. In-house core group research will be concerned with applications of FT-ICR-mass spectrometry to: a) peptide and protein sequencing, b) hydrogen-deuterium exchange in proteins, c) phospholipid identification and structural analysis, d) EPR spectroscopy of mass-selected trapped ions e) glow discharge FT-ICR for elemental analysis and depth profiling in semiconductors, and f) optical spectroscopy of trapped ions. User Facility: In the first year 50% of instrument time will be available to users outside Florida State University, increasing to 80% in subsequent years. Education Outreach: The facility will provide an outstanding training ground for st udents and other scientists in academic and industry. The program will include a large number of graduate and postdoctoral student, and visiting professors and industrial scientists. Intern programs will reach high school and undergraduate students and teachers. Representation by female and minority groups are specifically included. Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry is the most versatile technique for unscrambling and quantifying ion-molecule reaction kinetics and equilibria in the absence of solvent (i.e., the gas phase). Applications, range from protein structure to crude oil.

摘要建议：9413008 PI：Marshall，A。G.机构：佛罗里达州立大学该奖项来自化学研究工具和设施计划的奖项将有助于开发佛罗里达州立大学国家高磁场实验室的FT-ICR-MAS-MAS-MAS-MAS-MAS-MASS光谱设施。 使用该仪器进行的研究包括：直接测序，化学反应性以及对抗体大小的气相蛋白的形状分析（150,000 DA）；直接的光吸收光谱和质量选择的捕获离子的电子顺磁共振；以及常规的直接识别像原油一样复杂的混合物的未分离成分。 该设施将拥有世界上最高的ICR质谱仪。 FT-ICR的技术发展将对生物学和分析化学产生重大影响，NSF中心将增强这些发展对广泛用户社区的传播。 该设施的方法开发将包括：a）内部和外部电离技术，b）热图和/或固体化合物的离子源，c）IR激光解吸（LD）和D）高沸点混合物的蒸发。 In-house core group research will be concerned with applications of FT-ICR-mass spectrometry to: a) peptide and protein sequencing, b) hydrogen-deuterium exchange in proteins, c) phospholipid identification and structural analysis, d) EPR spectroscopy of mass-selected trapped ions e) glow discharge FT-ICR for elemental analysis and depth profiling in semiconductors, and f) optical被困离子的光谱。 用户设施：在第一年，仪器时间的50％将提供给佛罗里达州立大学以外的用户，随后几年增加到80％。 教育宣传：该设施将为学术和工业中的ST UDENT和其他科学家提供出色的培训理由。 该计划将包括大量的研究生和博士后学生，以及访问教授和工业科学家。 实习计划将访问高中，本科生和老师。 专门包括女性和少数群体的代表。 傅立叶转化离子回旋共振（FT-ICR）质谱法是在没有溶剂（即气相）的情况下取消和量化离子 - 分子反应动力学和平衡的最通用技术。 应用，从蛋白质结构到原油。