RUI: Molecular Regulation of Sodium Transport Systems in Euryhaline Animals

RUI：广盐动物钠转运系统的分子调控

基本信息

批准号：
9407261
负责人：
David Towle
金额：
$ 27.25万
依托单位：
Lake Forest College
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1994
资助国家：
美国
起止时间：
1994-07-15 至 1998-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9407261&HistoricalAwards=false
关键词：
RUI Molecular Regulation Sodium Transport

项目摘要

9407261 Towle Euryhaline animals by definition are capable of tolerating a broad range of salt levels in their aquatic environments. An example is the green shore crab Carcinus maenas, an introduced species in many intertidal habitats around the world. This crab succeeds in a range of environmental salinities by regulating its own salt levels in the blood, depending on transport systems in its multifunctional gills. Two sodium transporters in crab gill, a sodium/hydrogen exchanger and a sodium/potassium pump, are very similar to transporters in the mammalian kidney. If we can learn more about how the green crab and other euryhaline animals cope with varying salt loads under natural conditions, perhaps we will better understand processes in our own kidney which may be related to hypertension and other disorders of homeostasis. We may also learn enough about the specifics of the physiology of Carcinus to suggest environmentally safe approaches to its control as a pest species. Dr. Towle plans to study the two sodium transporters in the crab using the techniques of molecular biology. He will identify the genetic code for each of the transporters using the polymerase chain reaction (PCR). Determining the DNA sequence of each will permit a comparison with similar transporters in mammals and other vertebrates. He will also be able to study the molecular expression of each transporter in different specialized tissues under various salinity challenges. He expects that the transporters will be expressed most strongly in gills of animals challenged with low environmental salt, conditions in which salt uptake would be adaptive. The research will be accomplished with the assistance of undergraduates at Lake Forest College and Mount Desert Island Biological Laboratory. ***

根据定义，9407261拖曳Euryhaline动物能够耐受水生环境中的盐分范围。一个例子是绿色海岸蟹卡氏菌（Carcinus Maenas），这是世界各地许多潮间带栖息地中引入的物种。该蟹通过调节其血液中的盐水平，取决于其多功能ill的运输系统，从而在一系列环境盐分中取得成功。螃蟹吉尔中的两个钠转运蛋白，钠/氢交换剂和钠/钾泵在哺乳动物肾脏中的转运蛋白非常相似。如果我们可以进一步了解有关绿蟹和其他Euryhaline动物如何应对自然条件下的盐负荷，也许我们会更好地了解自己的肾脏中的过程，这可能与高血压和其他稳态疾病有关。我们还可以了解癌的生理学细节，以提出环境安全的方法来控制其作为害虫物种。 Towle博士计划使用分子生物学技术研究螃蟹中的两个钠转运蛋白。他将使用聚合酶链反应（PCR）确定每个转运蛋白的遗传密码。确定每个序列的DNA序列将允许与哺乳动物和其他脊椎动物中的相似转运蛋白进行比较。在各种盐度挑战下，他还将能够研究不同专用组织中每个转运蛋白的分子表达。他预计，转运蛋白将在面对低环境盐挑战的动物g中表达最强的表达，其中盐吸收会具有适应性。这项研究将在森林湖学院和荒岛山生物实验室的大学生的帮助下完成。 ***