Upgrading the Optical Facilities of the Biology Department at the City College of the City University of New York

纽约城市大学城市学院生物系光学设施升级

• 批准号: 9317925
• 负责人: John Lee
• 金额: --
• 依托单位: CUNY City College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-15 至 1997-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9317925&HistoricalAwards=false
• 关键词: Upgrading; Optical; Facilities; Biology; Department

Biologists at the City College of New York are requesting matching funds to up- gradeand provide new optical facilities for their research. Requested are funds to purchase: 1) a transmission electron microscope (TEM); 2) an inverted epifluorescent microscope; and 3) a confocal laser scanning microscope. We also wish to upgrade the stage of a constantly used digital scanning electron microscope (DSEM).The new TEM will replace the present TEM, a Phillips 300 purchased 21 years ago, which has limited value as a research instrument because it is not capable of producing images required to answer many contemporary research questions. At present we have strong need for an inverted epifluorescent microscope and a confocal laser scanning microscope. The present stage on our Zeiss digital scanning microscope is a bottle neck for effective use of this instrument. The present stage takes only one specimen at a time; we must pump down between specimen changes. The new stage holds 5 stubs (specimen holders) at a time. It also is automated and digitized. This will allow researchers to make montages. It will also give researchers, studying small organisms and cells, the ability to mark positions of specimens of interest by storage in memory so that the best specimens can be relocated, studied, and photographed. The research aided by the new equipment is quite broad in aspect, covering fields such as Neurobiology, Molecular Developmental Genetics, Immunobiology, Protistology, Molecular Systematics, Endocytobiology, and Evolution. More specifically the following projects will be aided: 1) Phagosome- phagolysosomal processing in diatom- bearing endosymbioses in foraminifera; 2)Karyokinesis and systematics of foraminifera; 3) Molecular systematics of planktonic diatoms; 4) Ce ll surface phenotypes of thymic nurse cells; 5) Contact between thymocytes and thymic nurse cells; 6) Molecular and genetic analysis of the Drosophila compound eye; 8) Molecular mechanisms underlying axonal development and connection specificity in the embryonic and metamorphosing nervous system of the frog Xenopus Iclevis; and 9) Studies of growth factors and neurotransmitters involved in the signal cascade by means of which the retina modulates growth of the sclera which, when awry, can lead to myopia. The City College is located in theHarlem section of New York City.and its student body is predominantly minority. Many students are involved with the proposed investigators in undergraduate and graduate research. Should this proposal be funded, there is every expectation that minority students will be involved in the research itself, and the fallout which enters other aspects of coursework and training.

纽约城市学院的生物学家正在申请配套资金，为他们的研究升级并提供新的光学设施。请求资金用于购买： 1) 透射电子显微镜 (TEM)； 2）倒置落射荧光显微镜； 3) 共焦激光扫描显微镜。我们还希望升级经常使用的数字扫描电子显微镜 (DSEM) 的载物台。新的 TEM 将取代目前的 TEM，即 21 年前购买的 Phillips 300，它作为研究仪器的价值有限，因为它无法生成回答许多当代研究问题所需的图像。目前我们对倒置落射荧光显微镜和共焦激光扫描显微镜有强烈的需求。我们的蔡司数字扫描显微镜的现阶段是有效使用该仪器的瓶颈。现阶段一次只采集一个样本；我们必须在更换样本之间抽真空。 新的载物台可同时容纳 5 个存根（样本架）。它也是自动化和数字化的。这将使研究人员能够制作蒙太奇。它还将使研究小型生物体和细胞的研究人员能够通过存储在内存中来标记感兴趣样本的位置，以便可以重新定位、研究和拍摄最好的样本。 新设备辅助的研究领域相当广泛，涵盖神经生物学、分子发育遗传学、免疫生物学、原生生物学、分子系统学、内细胞生物学和进化等领域。更具体地说，以下项目将得到帮助：1）有孔虫中硅藻内共生体的吞噬体-吞噬溶酶体加工； 2）有孔虫核分裂与系统学； 3）浮游硅藻的分子系统学； 4) 胸腺滋养细胞的细胞表面表型； 5) 胸腺细胞和胸腺滋养细胞之间的接触； 6）果蝇复眼的分子和遗传分析； 8) 青蛙 Xenopus Iclevis 胚胎和变态神经系统中轴突发育和连接特异性的分子机制； 9) 研究参与信号级联的生长因子和神经递质，视网膜通过信号级联调节巩膜的生长，当巩膜出现异常时，可能导致近视。 城市学院位于纽约市的哈莱姆区，其学生主体以少数族裔为主。许多学生参与了本科生和研究生研究中拟议的研究人员。如果这项提案获得资助，那么人们完全期望少数族裔学生能够参与研究本身，以及影响课程和培训其他方面的内容。