Optical Analysis of Synaptic Integration in Cerebellar Purkinje Cells

小脑浦肯野细胞突触整合的光学分析

基本信息

批准号：
9209784
负责人：
William Ross
金额：
$ 20.69万
依托单位：
New York Medical College
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
1992
资助国家：
美国
起止时间：
1992-09-01 至 1996-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9209784&HistoricalAwards=false
关键词：
Optical Analysis Synaptic Integration Cerebellar

项目摘要

This application makes use of state-of-the-art imaging techniques to spatially and temporally resolve the propagation of specific ionic transients. Dr. Ross will investigate this problem by using a relatively noninvasive high speed imaging system which can examine all parts of the cell simultaneously, This optical technique can give information about specific ion concentration changes and voltage changes. The signals can be interpreted in terms of the spatial distribution and propagation of dendritic events and the distribution of specific membrane channels. Dr. Ross will inject individual Purkinje cells in cerebellar slices with fluorescent ion indicators and high speed images, correlated with simultaneous electrical measurements made under various conditions. The special imaging technology will be specifically used for measuring intracellular calcium and sodium concentration transients in fine dendritic processes. These experiments will attempt to define those factors (intrinsic membrane conductances, synaptic interactions, and neuromodulators) which influence the generation of local intracellular calcium changes and the propagation of regenerative potentials. Because it is essential to have information about the way synaptic inputs are integrated and processed in a neuron results from this study will ultimately help to better understand the cellular mechanisms of learning and memory.***

该应用利用最先进的成像技术在空间和时间上解决特定离子瞬变的传播。罗斯博士将通过使用相对无创的高速成像系统来研究这个问题，该系统可以同时检查细胞的所有部分，这种光学技术可以提供有关特定离子浓度变化和电压变化的信息。这些信号可以用树突事件的空间分布和传播以及特定膜通道的分布来解释。罗斯博士将在小脑切片中注入单个Purkinje细胞，并带有荧光离子指示器和高速图像，与在各种条件下进行的同时进行电气测量有关。特殊的成像技术将专门用于测量细胞内钙和钠浓度瞬变，并在精细的树突过程中。这些实验将尝试定义这些因素（固有的膜电导，突触相互作用和神经调节剂），这些因素会影响局部细胞内钙变化的产生和再生电位的传播。因为重要的是要获得有关突触输入的方式进行整合和处理在这项研究的结果中的信息，最终将有助于更好地了解学习和记忆的细胞机制。***