Regulation of Gonadotropin Gene Expression by Inhibin Activin and GnRH

抑制素激活素和 GnRH 对促性腺激素基因表达的调节

• 批准号: 9207298
• 负责人: Barbara Attardi
• 金额: $ 12.8万
• 依托单位: University of Pittsburgh
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-15 至 1995-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9207298&HistoricalAwards=false
• 关键词: Regulation; Gonadotropin; Gene; Expression; Inhibin

The pitutary gonadotropin hormones, luteinizing hormone (LH) and follicle stimulating hormone (FSH), play an essential role in mammalian development and normal reproductive function. These two gonadotropin hormones are composed of two subunits, a common alpha-subunit and a beta-subunit which is unique and confers specificity. Dr. Attardi has demonstrated that gonadotropin releasing hormone, a hormone found in the hypothalamus of the brain, and the gonadal hormones, inhibin, activin and follistatin, cause rapid and profound effects on the synthesis of FSH but not LH. These changes are mediated through alternation in the messenger RNA which codes for the beta-subunit of the FSH protein. Dr. Attardi will examine whether these effects result from changes in synthesis rate of messanger RNA for FSH beta-subunit or from changes in stability of the FSH beta-subunit molecules. The results from these studies will help elucidate how hypothalamic and gonadal hormones regulate gonadotropin synthesis and secretion. This will increase our understanding of how pituitary cells communicate among themselves as well as with hypothalamic neurons and cells in the ovaries and testes. Moreover, the results could lead to the development of safer and more effective methods of fertility control and increased ability to diagnose and treat problems of infertility.

尖锐的促性腺激素激素，黄体激素（LH）和卵泡刺激激素（FSH）在哺乳动物发育和正常生殖功能中起着至关重要的作用。 这两种促性腺激素激素由两个亚基组成，一个共同的α-亚基和一个独特且具有特异性的β-亚基。 Attardi博士证明，促性腺激素释放激素，一种在大脑下丘脑中发现的激素，以及促性腺激素，抑制素，激活素和卵泡素对FSH的合成，但没有LH，从而快速而深远地影响。 这些变化是通过信使RNA中的交替介导的，该信使RNA代码为FSH蛋白的β-亚基编码。 Attardi博士将检查这些影响是由于Messanger RNA的合成速率的变化而导致的，是FSHβ-亚基的合成速率，还是FSHβ-亚基分子的稳定性变化。 这些研究的结果将有助于阐明下丘脑和性腺激素如何调节促性腺激素的合成和分泌。 这将提高我们对垂体细胞之间以及卵巢和睾丸中下丘脑神经元和细胞之间如何交流的理解。 此外，结果可能会导致发展更安全，更有效的生育能力控制方法，并提高诊断和治疗不育问题的能力。