Biomolecular Sol-Gel Glasses Containing Encapsulated Enzymesand Other Proteins

含有封装酶和其他蛋白质的生物分子溶胶-凝胶玻璃

• 批准号: 9202182
• 负责人: Bruce Dunn
• 金额: $ 41.4万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-01 至 1995-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9202182&HistoricalAwards=false
• 关键词: Biomolecular; Sol; Gel; Glasses; Containing

This research program is at the interface between materials science and chemistry/biochemistry. The collaborative research effort is aimed at developing a new type of biomolecular material in which enzymes and other proteins are encapsulated in inorganic glass matrices synthesized by the sol-gel method at room temperature. Biochemistry is largely a solution-based discipline, but the application of the sol-gel technique enables us to prepare novel solid-state materials which exhibit the electrical, optical, chemical and biological properties of the biomolecule as well as the stability and integrity of the inorganic oxide matrix. The primary objectives of this project are (1) to develop methods of synthesis for this new class of solid-state biomolecular materials, (2) to elucidate the nature of the interactions between the glasses and the biomolecules and (3) to explore applications of these materials as optically-based biosensors. The research results are expected to provide a fundamental understanding of the role of synthesis conditions in retaining the properties of the biomolecule dopant in the solid-state environment. The optical transparency of the matrix enables us to use spectroscopic methods to characterize the structure, chemical function and activity of the biomolecule dopant. The work on biosensors is designed to exploit the specificity of enzymes and other proteins in combination with the transparent, porous and chemically stable matrix of the inorganic oxide. This Materials Synthesis and Processing project is jointly funded by the NSF Divisions: The Division of Materials Research; Division of Molecular and Cellular Biosciences; Division of Chemistry.

该研究项目处于材料科学和化学/生物化学之间的交叉领域。 该合作研究工作旨在开发一种新型生物分子材料，其中酶和其他蛋白质被封装在通过溶胶-凝胶法在室温下合成的无机玻璃基质中。 生物化学很大程度上是一门基于溶液的学科，但溶胶-凝胶技术的应用使我们能够制备新型固态材料，这些材料表现出生物分子的电学、光学、化学和生物特性以及生物分子的稳定性和完整性。无机氧化物基质。 该项目的主要目标是（1）开发此类新型固态生物分子材料的合成方法，（2）阐明玻璃与生物分子之间相互作用的本质，以及（3）探索应用这些材料作为基于光学的生物传感器。 研究结果有望为合成条件在固态环境中保留生物分子掺杂剂的特性方面的作用提供一个基本的了解。 基质的光学透明度使我们能够使用光谱方法来表征生物分子掺杂剂的结构、化学功能和活性。 生物传感器的工作旨在利用酶和其他蛋白质与透明、多孔和化学稳定的无机氧化物基质的结合的特异性。 该材料合成与加工项目由 NSF 部门联合资助：材料研究部；分子和细胞生物科学部；化学系。