Bio-Imaging Analyzer (Phosphorimager)

生物成像分析仪（Phosphorimager）

• 批准号: 9110640
• 负责人: Paul Matsudaira
• 金额: $ 5.5万
• 依托单位: Whitehead Institute for Biomedical Research
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-06-01 至 1994-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9110640&HistoricalAwards=false
• 关键词: Bio; Imaging; Analyzer; Phosphorimager

This proposal involves a request to purchase the Fuji Bio-Imaging Analyzer (Phosphorimager), a new device for the detection and quantitation of radioactivity used in a variety of biochemical experiments. The quantitative capabilities of this instrument will allow biochemical experiments not currently possible. The primary advantages of this technology over conventional autoradiography using x-ray film are an increased linear response, dynamic range, and sensitivity, as well as reduced exposure time. The instrument provides quantitative data from experiments involving the use of this radioisotopes such as 32P, 35S, 14C, and 125I. An instrument of this type is not currently available at the Whitehead Institute. The quantitation of radioactive samples will be carried out by the user groups in a variety of biochemical and molecular biology experiment detailed in Section 8. The technology involves the use of storage phosphor screens which store energy form sample beta particle emission. This emission is detected by the instrument and stored on a computer hard disk. The data can then be accessed and analyzed using a software package provided with the instrument. A number of quantitative biochemical experiments not currently possible will be feasible because the Phosphorimager is vastly superior to X-ray film autoradiography.

该提案涉及购买富士生物成像分析仪（Phosphorimager）的请求，这是一种用于检测和定量各种生化实验中使用的放射性的新设备。 该仪器的定量能力将允许进行目前不可能的生化实验。与使用 X 射线胶片的传统放射自显影技术相比，该技术的主要优点是增加了线性响应、动态范围和灵敏度，以及减少了曝光时间。 该仪器提供涉及使用此类放射性同位素（例如 32P、35S、14C 和 125I）的实验的定量数据。 怀特海研究所目前还没有这种类型的仪器。 放射性样品的定量将由用户组在第 8 节中详细介绍的各种生化和分子生物学实验中进行。该技术涉及使用存储荧光屏，该荧光屏存储样品 β 粒子发射的能量。 该发射由仪器检测并存储在计算机硬盘上。 然后可以使用仪器附带的软件包访问和分析数据。 由于 Phosphorimager 远远优于 X 射线胶片放射自显影，因此目前无法进行的许多定量生化实验将变得可行。