Domains of Gene Activity in Developing Embryos of Brassica napus

甘蓝型油菜胚胎发育中的基因活性域

• 批准号: 9105527
• 负责人: Donna Fernandez
• 金额: $ 27.6万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 1995-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9105527&HistoricalAwards=false
• 关键词: Domains; Gene; Activity; Developing; Embryos

The goal of the proposed research is to further our understanding of the role spatial information plays in regulating embryo development and the transition to germination in higher plants. The approach will be to use molecular probes and in situ localization techniques to look at the cellular basis for patterns of gene expression in Brassica napus embryos, and to examine how these patterns change over the course of development. An important consequence of this research will be there identification of clones that can be used to look at the molecular anatomy of the processes that appear to involve pattern formation, particularly the determination of the shoot apical meristem during early embryogenesis and the determination of organ identity at the shoot apex during precocious germination. These clones can also be used, in combination with genetic analysis of developmental mutants in Arabdopsis to dissect the mechanisms of pattern formation in plant embryos.

拟议研究的目的是进一步了解空间信息在调节高等植物胚胎发育和发芽过渡中的作用。该方法将使用分子探针和原位定位技术来研究甘蓝型油菜胚胎中基因表达模式的细胞基础，并检查这些模式在发育过程中如何变化。 这项研究的一个重要结果将是鉴定克隆，这些克隆可用于观察涉及模式形成的过程的分子解剖学，特别是早期胚胎发生过程中茎尖分生组织的确定和早期胚胎发生过程中器官身份的确定。早熟发芽期间的芽尖。 这些克隆还可以与拟南芥发育突变体的遗传分析结合使用，以剖析植物胚胎中模式形成的机制。