Undergraduate Patch Clamp Labs on Single Ion Channel Electrophysiology in Cell Membranes

细胞膜单离子通道电生理学本科膜片钳实验室

• 批准号: 8852265
• 负责人: Kent Chapman
• 金额: $ 1.84万
• 依托单位: Brown University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-12-15 至 1991-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8852265&HistoricalAwards=false
• 关键词: Undergraduate; Patch; Clamp; Labs; Single

This award to Brown University will provide funds to equip an undergraduate Cell Physiology and Biophysics course, and two undergraduate Independent Study and Honors research project courses, with patch clamp amplifiers, computer interfaces and software for experiments on the electrophysiology of single ion channels in the plasma membranes of excitable cells. Patch clamp recording is a revolutionary electrophysiological technique developed in the late 1970's and early 1980's. It has taken cellular electrophysiology to the molecular level, and is rapidly creating an important new integration of traditionally disparate fields. These funds will provide equipment for 8 workstations, accommodating up to 32 students working in pairs, in split sessions of 16 students each. The major expenses are for interfaces to 8 existing computers, the requisite computer software, and 5 patch amplifiers to supplement 3 already available. Students will learn basic technical skills, the characteristics of representative ion channels, theoretical principles of interpreting channel behavior in terms of likely mechanisms, and an appreciation of significant biological problems that have become tractable by this approach. This project would also significantly upgrade the current teaching equipment from 1960's vacuum tube technology to the lab computer era.

该奖项授予布朗大学，将为本科生细胞生理学和生物物理学课程以及两门本科生独立研究和荣誉研究项目课程提供资金，配备膜片钳放大器、计算机接口和用于等离子体中单离子通道电生理学实验的软件可兴奋细胞的膜。 膜片钳记录是 20 世纪 70 年代末和 80 年代初开发的革命性电生理技术。 它将细胞电生理学提升到分子水平，并正在迅速创造传统不同领域的重要新整合。 这些资金将为 8 个工作站提供设备，最多可容纳 32 名学生，两人一组，每班 16 名学生。 主要费用是与 8 台现有计算机的接口、必需的计算机软件以及 5 个贴片放大器（用于补充 3 个现有计算机）。 学生将学习基本的技术技能、代表性离子通道的特征、根据可能的机制解释通道行为的理论原理，以及对通过这种方法可以解决的重要生物学问题的理解。 该项目还将当前的教学设备从 1960 年代的真空管技术显着升级到实验室计算机时代。