Molecular targets and actions of ethanolamide-conjugated oxylipins in Arabidopsis thaliana

拟南芥中乙醇酰胺结合的氧脂质的分子靶点和作用

基本信息

批准号：
1656263
负责人：
Kent Chapman
金额：
$ 68.29万
依托单位：
University of North Texas
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-03-15 至 2021-02-28
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1656263&HistoricalAwards=false
关键词：
Molecular targets actions ethanolamide conjugated

项目摘要

Plants use small molecules as signals to synchronize their growth, development and responses to their environment. This proposal seeks to understand how seedlings use chemical signals to modulate their growth in response to adverse environmental conditions, a response that allows them to endure environmental stress at a time when they are most vulnerable. Specific research aims include the identification of mechanisms for signal perception, the metabolic fate of signals in seedlings after perception, and the discovery of the signaling networks activated during seedling stress. Broader applications of this research include the potential for designing new breeding targets in crops for improved field emergence. In addition, the proposed research activities are integrated with undergraduate training opportunities through a newly-designed research course at the University of North Texas, a large minority-serving, public university. N-Acylethanolamines (NAEs) are ethanolamide-conjugated fatty acid derivatives that in animal systems include the widely-recognized bioactive endogenous metabolites of the "endocannabinoid signaling pathway". This pathway regulates an array of physiological and behavioral processes in mammals, but despite the documented occurrence of NAEs in plant systems, their physiological actions remain incompletely understood. Recent evidence indicates that oxylipin derivatives of polyunsaturated NAEs have potent biological activities in both animal and plant systems, and in plants this may be one major route of action for NAEs. This proposal aims to expand the understanding of this group of bioactive lipids and their role as negative regulators of seedling growth in the model plant, Arabidopsis thaliana, through the following three specific aims: 1) Identify NAE oxylipin target proteins with biotinylated probes; 2) Determine if NAE oxylipins are substrates for the fatty acid amide hydrolase (FAAH); 3) Delineate the spatial interaction of NAE oxylipins with ABA- and G-protein signaling in roots and shoots of seedlings. Results from this research may ultimately lead to new targets for the improvement of crop emergence under environmental stress, and may also offer new strategies for reduction of competing weed emergence in early season crop establishment. In addition to the potential broader applications, this project will offer training opportunities for undergraduate students through a newly-designed research course at the University of North Texas-- Advanced Research in Life Sciences (BIOL 3900).

植物使用小分子作为信号，以同步其生长，发展和对环境的反应。该提案试图了解幼苗如何使用化学信号来调节其对不利环境条件的生长，这种反应使他们能够在最脆弱的时候忍受环境压力。具体研究的目的包括鉴定信号感知机制，感知后幼苗信号的代谢命运以及在幼苗应激期间激活的信号网络的发现。这项研究的更广泛的应用包括在农作物中设计新的育种靶标的潜力，以改善田间出现。此外，拟议的研究活动与北德克萨斯大学（University of North Dexas）的新设计的研究课程（一所大型公立大学）通过新设计的研究课程融入了本科培训机会。 N-酰基乙醇胺（NAES）是乙醇胺偶联的脂肪酸衍生物，在动物系统中，包括“内源性大麻素信号通路”的广泛认可的生物活性内源性代谢产物。该途径调节了哺乳动物中的一系列生理和行为过程，但是尽管有记录在植物系统中发生NAE，但其生理作用仍未完全理解。最近的证据表明，多不饱和NAES的催产素衍生物在动物和植物系统中具有有效的生物学活性，而在植物中，这可能是NAES的主要作用途径。该建议旨在通过以下三个特定目的扩大对模型植物拟南芥中幼苗生长的负调节剂的作用，以扩大对这组生物活性脂质的理解及其作用：1）鉴定使用生物素化探针的NAE OXYLIPIN靶蛋白； 2）确定NAE Oxylipins是否是脂肪酸酰胺水解酶（FAAH）的底物； 3）描绘了幼苗根和芽中的NAE Oxylipins与ABA-和G蛋白信号传导的空间相互作用。这项研究的结果最终可能导致新的目标，以改善环境压力下的作物出现，并且还可能为减少早期作物建立的杂草出现的新策略提供了新的策略。除了潜在的更广泛的应用外，该项目还将通过北德克萨斯大学（University of North Dexas）的新设计的研究课程为本科生提供培训机会 - 生命科学的高级研究（BIOL 3900）。