Enzymology of DNA Repair, SFC Award in Pakistani and U.S. Currencies

DNA 修复酶学，巴基斯坦和美国货币 SFC 奖

• 批准号: 8713991
• 负责人: Lawrence Grossman
• 金额: $ 4.63万
• 依托单位: Johns Hopkins University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-08-15 至 1991-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8713991&HistoricalAwards=false
• 关键词: Enzymology; DNA; Repair; SFC; Award

Description: This project is for research on the molecular mechanisms of cellular repair of alkylated DNA in M. Iuteus after its exposure to alkylating agents. The experiments will involve purification and characterization of enzymes which are seemingly specific to certain alkylating agents. Their structural chemistry will be studied in order to investigate the physiochemical interaction between repair proteins and DNA base adducts. These experiments will be carried out by Dr. A. Athar at the Center for Advanced Molecular Biology (CAMB) in Lahere, Pakistan. The laboratory of Dr. Lawrence Grossman at Johns Hopkins University will assist Pakistani collaborators in conducting those experiments that could not be done in Pakistan. Scope: The collaboration is beneficial to both institutions and is expected to yield information leading to better understanding of molecular mechanisms of cellular repair of alkylation damage to genetic material. The past work by the two laboratories has resulted in several joint publications.

描述：该项目旨在研究 M. Iuteus 暴露于烷化剂后烷基化 DNA 细胞修复的分子机制。 实验将涉及酶的纯化和表征，这些酶似乎对某些烷化剂具有特异性。 将研究它们的结构化学，以研究修复蛋白和 DNA 碱基加合物之间的理化相互作用。 这些实验将由位于巴基斯坦拉赫尔的高级分子生物学中心 (CAMB) 的 A. Athar 博士进行。 约翰·霍普金斯大学劳伦斯·格罗斯曼博士的实验室将协助巴基斯坦合作者进行那些在巴基斯坦无法完成的实验。 范围：此次合作对两个机构都有好处，预计将产生信息，从而更好地理解细胞修复遗传物质烷基化损伤的分子机制。 两个实验室过去的工作已经发表了几篇联合出版物。