RUI: Acquisition of a DNA Synthesizer System

RUI：获得 DNA 合成器系统

• 批准号: 8706678
• 负责人: Judith Zyskind
• 金额: $ 4万
• 依托单位: San Diego State University Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-15 至 1990-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8706678&HistoricalAwards=false
• 关键词: RUI; Acquisition; DNA; Synthesizer; System

The principal investigator has requested funds for the purchase of a DNA Synthesizer System. The equipment will be shared by five investigators who use recombinant DNA techniques and whose research programs are in critical need of on-site oligonucleotide synthesis capability to produce primers for DNA synthesis and in vitro mutagenesis. The synthesizer will be utilized initially in projects that are concerned with the molecular genetics of the Drosophila myosin heavy chain gene; enzyme degradation in mammalian erythroid cells; regulation of RNA virus gene expression; expression of genes encoding the small nuclear RNAs; and initiation of DNA replication in E. coli. The instrumentation will directly benefit the San Diego State University's techniques increases and students gain familiarity with current technology.

首席研究员已请求资金购买 DNA 合成器系统。 该设备将由五名使用重组 DNA 技术的研究人员共享，他们的研究项目迫切需要现场寡核苷酸合成能力，以产生用于 DNA 合成和体外诱变的引物。 该合成器最初将用于与果蝇肌球蛋白重链基因的分子遗传学有关的项目；哺乳动物红细胞中的酶降解； RNA病毒基因表达的调控；编码小核RNA的基因的表达； 以及大肠杆菌中 DNA 复制的启动。 该仪器将直接有利于圣地亚哥州立大学的技术进步和学生熟悉当前技术。