MRI/RUI: Acquisition of Genetic Analyzer and Sequence Detection System for the Sonoma State University Core DNA Analysis Facility

MRI/RUI：为索诺玛州立大学核心 DNA 分析设施购置遗传分析仪和序列检测系统

• 批准号: 0321367
• 负责人: Derek Girman
• 金额: --
• 依托单位: Sonoma State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0321367&HistoricalAwards=false
• 关键词: MRI; RUI; Acquisition; Genetic; Analyzer

This project, upgrading capabilities of a Core DNA Analysis Facility, builds on a successful NSF CCLI award. The acquisition of a capillary based genetic analyzer and a real-time PCR detection system is proposed to complete investigations in a variety of fields ranging from studies of gene regulation, to the mentoring and modeling of disease spread, to molecular systematics, and conservation genetics. Ten of the on-going projects use DNA sequencing for various purposes, including molecular systematics, mapping of phenotypic characters, examination of population structure, understanding biogeography, and identification and characterization of specific genes. Of four genotyping projects, three use analysis of highly variable microsatellites for fine scale population analyses, analyses of kinship (genetic fingerprinting), or mutation detection. One genotyping project uses AFLP analysis to conduct genome-wide sampling for use in GIS modeling that includes a genotypic "landscape." Two projects rely simply on genotype detection or mutation detection by analysis of the presence/absence or condition of PCR products. In line with the mission to foster combined research/education, this work carries a good success story.The project, accommodating the increased demand for usage of the facility by students and faculty, not only enhances faculty research programs, but also involves research training in an undergraduate institution. Moreover, teacher training is expected to impact on K-12 education.

该项目升级了核心 DNA 分析设施的能力，以成功的 NSF CCLI 奖项为基础。建议购买基于毛细管的遗传分析仪和实时 PCR 检测系统，以完成从基因调控研究到疾病传播的指导和建模、分子系统学和保护遗传学等各个领域的研究。十个正在进行的项目将 DNA 测序用于各种目的，包括分子系统学、表型特征绘图、群体结构检查、了解生物地理学以及特定基因的识别和表征。在四个基因分型项目中，三个利用高度可变的微卫星分析进行精细规模的群体分析、亲属关系分析（基因指纹）或突变检测。一个基因分型项目使用 AFLP 分析进行全基因组采样，用于包括基因型“景观”的 GIS 建模。两个项目仅依赖于通过分析 PCR 产物的存在/不存在或状况来进行基因型检测或突变检测。本着促进研究/教育相结合的使命，这项工作取得了良好的成功。该项目满足了学生和教师对设施使用日益增长的需求，不仅加强了教师的研究项目，而且还涉及以下领域的研究培训：本科院校。此外，教师培训预计也会对 K-12 教育产生影响。