基于移频机制的宽场非标记超分辨成像新方法研究

Label-free super resolution in visible-band is of significant importance to the development of biological cytology, materials science and micro-electronics etc. One of the biggest challenges in optical microscopy is is how to realize wide-field wide-band super-resolution. In this project, based on the frequency-shift effect, a novel super resolution method has been proposed. By shifting the high spatial frequency of the sample with different amount, corresponding imaging message will be gotten. Then with iterative reconstruction, we can realize a label-free wide-field and far-field super-resolution imaging. We will study the modulation on nano illumination beam and construct a super resolution chip for nanoscopy, which can be easily integrated with conventional microscope. Combing with optimization algorithm, we will suppress the background noise and improve the signal-to-noise ratio. We will also carry out the research on phase imaging technology, which will realize the longitudinal super-resolution and three dimensional super-resolution. This project will provide a new way for the existing optical super-resolution imaging technologies.

可见光波段亚波长非荧光样品纳米成像技术对于生物细胞学、材料科学和微电子等领域的发展具有重要意义。如何对非标记样品实现大视场宽频段的超分辨成像是光学显微技术未来需要解决的关键难点问题之一。本项目基于前期移频效应的研究，提出了一种新型超分辨显微成像方法，通过对微纳样品高分辨信息对应的空间频率大范围频移，从显微物镜获取不同频移量下的成像信息，在傅里叶频谱域迭代拼接，重构超分辨图像，实现对微纳样品非标记、宽场、远场的超分辨成像。项目将通过对微纳光源的光束综合调控研究，构建超分辨芯片，方便与普通显微成像系统集成，结合优化处理算法，获取高信噪比成像效果，实现对微纳样品的超分辨成像。发展移频成像的相位高分辨成像方法，实现对微纳样品纵向高分辨信息的获取，结合宽场大范围移频成像探索三维超分辨成像的新方法。本项目的实施将为现有光学超分辨技术三维成像提供一种全新的思路。