香港牡蛎和熊本牡蛎高密度遗传连锁图谱构建及其杂交子代生长、糖原含量相关QTL定位研究

Crassostrea hongkongensis and C.sikamea are two mollusk species of economic importance in China. Like other agricultural species, new oyster strains with favorable traits are desired for promoting the sustainable development of oyster cultivation industry. Therefore, development of the tools and methods using marker-assisted breeding technology are very important and definitely needed for breeding program. This project is based on the obtaining of hybrid families of C. hongkongensis and C.sikamea, aims to screen a large number of SNP markers that can be amplified in both oyster by Reduced-Representation Genome Sequencing 2b-RAD, and construct high density genetic linkage maps for C. hongkongensis and C. sikamea using SNP and SSR markers. In the same time, this project will map growth and glycogen content related QTLs in hybrid progeny populations and investigate the potential value of these QTL in selective breeding program of the oysters. The results would provide theoretical and technical basis for the marker-assisted breeding technology system in oysters.

香港牡蛎和熊本牡蛎是我国两种重要的海水经济贝类，近年来随着牡蛎产业规模的不断扩大，急需培育具有优良性状的牡蛎新品种促进我国牡蛎养殖业持续健康的发展，因此尽快开展分子标记辅助育种研究具有重要的经济价值和理论意义。本项目在成功获得香港牡蛎和熊本牡蛎杂交家系的基础上，拟采用简化基因组测序2b-RAD技术，大规模筛选可同时在两种牡蛎中扩增的SNP标记，并结合从基因组和转录组中开发的微卫星标记，构建两种牡蛎的雌、雄高密度遗传连锁图谱和一致性图谱；同时利用杂交子代对生长、糖原含量相关QTL进行定位研究，并探讨这些QTL或标记在牡蛎良种培育中的应用价值。研究成果将为发展分子标记辅助育种技术体系、培育优良牡蛎新品种提供理论依据和技术基础。