草珊瑚生长与药用成分合成的光调控机制

结题报告

项目介绍

AI项目解读

基本信息

批准号：
32001246
项目类别：
青年科学基金项目
资助金额：
24.0万
负责人：
何茜
依托单位：
华南农业大学
学科分类：
森林信息学与森林经理学
结题年份：
2023
批准年份：
2020
项目状态：
已结题
起止时间：
2020 至 2023

项目参与者：
何茜；
关键词：
光环境生长特性药用成分关键酶活性光合生理草珊瑚

项目摘要

Light is a key factor that restricts the growth and development of understory medicinal plants. Light affects medicinal plants growth, morphology and the accumulation of secondary metabolites. Sarcandra glabra is an important medicinal plant in south China. At present, wild resources are exhausted. Adopting the method of understory wild cultivation can obtain great benefits. Previous studies have found that the biomass and the medicinal components of Sarcandra glabra varied by 5-10 times due to the difference of understory light environment. This difference may be related to the understory light intensity or light quality factor. However, the regulatory mechanisms of light intensity and light quality, on growth, physiological and medicinal components of Sarcandra glabra are still unclear. Therefore, this project through artificial lighting cultivation experiment, to explore light intensity and light quality how to affects the organ growth, biomass, leaf form, photosynthetic physiology, rosmarinic acid contents and flavonoids contents. Transcriptome high-throughput sequencing technology was used to analyze the key synthetic molecular regulation network of light on medicinal components of Sarcandra glabra, and to elucidate the light environment regulation mechanism of medicinal components. This study will provide an important theoretical basis for the light regulate the growth and the synthesis of medicinal ingredients of Sarcandra glabra, as well as a new idea to choose the light environment for the cultivation of understory Sarcandra glabra. It also provides the reference for the research and development of light formula in factory cultivation of medicinal plants.

光是制约林下药用植物生长发育的关键因素，对其生长、形态建成和次生代谢物质的积累等有很大影响。草珊瑚是南方地区的重要药用植物，目前野生资源面临枯竭，采取林下仿野生栽培的方式可获得较大收益。前期研究发现，不同林下光环境导致草珊瑚生物量及药用成分含量相差高达5-10倍，这种差异可能与林下光强或者光质因子有关。然而，光强和光质对草珊瑚生长、生理及药用成分合成的作用效果和调控机制尚不明确，因而无法证实以上推测。本项目通过人工控制光照试验，探究光强和光质因子对草珊瑚生长、叶片形态、生物量、光合生理及其药用活性成分迷迭香酸、总黄酮合成的影响，联合转录组测序技术解析光照对草珊瑚药用成分关键合成分子调控网络，阐明草珊瑚药用成分的光环境调控机制。研究将为草珊瑚生长与药用成分合成的光调控提供重要理论依据，也可为林下草珊瑚培育的光环境选择提供新的思路，同时为其他药用植物工厂化培育的人工光源研发提供数据支持。