低水平抗生素胁迫下微囊藻的生理生化及水处理行为特征

Cyanobacterial (e.g. Microcystis) blooms are serious environmental issue facing water supply and the maintenance of healthy water ways. Recently, a wide variety of antibiotics have been frequently detected in surface waters. Although the antibiotics detected are generally at low concentrations, they could have various impacts on Microcystis cells. Consequently, antibiotics may affect the effectiveness of control and water treatment processes on Microcystis management through affecting the cells, which would pose potential risks for drinking water safety. The approaches of this study are to systematically evaluate the impacts of antibiotics with environmentally relevant concentrations on Microcystis cells including cell density, cell integrity and cellular organic materials; to explore the genome-wide regulation mode and mechanisms of cellular resistance expression of Microcystis cells exposed to antibiotics using transcriptomics; and to investigate the action mechanisms of antibiotics on control (e.g., copper sulphate) and oxidation (e.g., potassium permanganate) techniques on Microcystis treatments. The above progresses achieved will reveal the molecular mechanisms of Microcystis cellular responses to anitibiotics. In addition, these observations will benefit the water authorities to optimize the technologies for Microcystis treatment where antibiotic is simultaneously present, and provide novel insights and scientific evidence for improvement of drinking water safety.

以微囊藻为代表的蓝藻水华对饮用水处理工艺的正常运行及水质安全造成严重威胁；而抗生素等水中频繁检出的痕量新兴污染物可能通过影响藻类生理生化特征，进而对水处理控藻/除藻工艺的效率产生潜在影响，增加饮用水安全隐患。本项目拟通过系列实验验证低水平（环境浓度）抗生素对微囊藻细胞密度、活性、胞内胞外有机物质等生理生化指标的影响；利用转录组技术揭示微囊藻细胞响应抗生素刺激的全局基因调控模式和环境胁迫耐性调整机制；深入探索抗生素刺激后微囊藻变化对后续控藻（如硫酸铜除藻剂）和水处理氧化藻（如高锰酸钾）效率的影响机制。本项目旨在揭示微囊藻应激水体抗生素的分子机制，并为优化抗生素污染水体中微囊藻的控制处理技术、提升饮用水安全性提供新的思路及科学依据。

以微囊藻为代表的蓝藻水华对饮用水处理工艺的正常运行及水质安全造成严重威胁；而抗生素等水中频繁检出的痕量新兴污染物可能通过影响藻类生理生化特征，进而对水处理控藻/除藻工艺的效率产生潜在影响，增加饮用水安全隐患。本研究结果通明确了了低水平（环境浓度）抗生素对微囊藻细胞具有毒物兴奋效应，对微囊藻水华形成具有显著影响；不同浓度抗生素作用后的微囊藻，对于微囊藻藻类有机质和伴生的藻毒素等，其产量和胞内外含量分布比例明显不同。本项目通过转录组技术揭示了微囊藻细胞响应抗生素刺激的全局基因调控模式和环境胁迫耐性调整机制。基于微囊藻在抗生素作用下生理生化特征的变化，本项目首次考察了抗生素刺激后微囊藻的变化对后续高锰酸钾工艺氧化藻源水效率的影响规律和相关机制。研究发现高锰酸钾灭活微囊藻和降解藻毒素的效率减弱，并提出抗生素作用后微囊藻藻毒素合成基因及细胞生长的变化，以及藻液中残留的抗生素等，都是降低高锰酸钾氧化效率的主要机制。本项目为优化抗生素污染水体中微囊藻的控制处理技术、提升饮用水安全性提供新的思路及科学依据。

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