关于花鲈种质资源及种群的遗传学与分类学研究

Sea bass is one of the main commercial fish in aquaculture. There are some quentions on systematic position of Chinese sea bass. The systematic evolution of germplasm resources, taxonomic and the genetic variation of sea bass were studied. The study were carried out by using morphological characteristics, isozyme analysis, RAPD, sequence comparison analysis. The results indicated that: there were obvious morphological differences between Chinese and Japanese sea bass. Significant differences existed in gill rakes, number of vertebrate and scales. A nearly complete replacement of alleles at LDH*, GPI-1*, GPI-2* locus between Japanese and Chinese sea bass. The Nei's genetic distance between Chinese and Japanese sea bass was much larger than that among Chinese sea bass populations. There were about 7% sequence differences at mtDNA cytb gene between them but no difference among Chinese sea bass individuals. Sequence comparison analysis of D-loop and S7 ribosome protein gene indicated that significant differences were also existed between Chinese and Japanese sea bass but little differences among Chinese sea bass populations or Japanese sea bass populations.

采用先进的现代生物技术(同工酶，线粒体DNA)，结合花鲈形态多变量分析，研究花鲈种质试吹南低辰掷啵秩阂糯湟煲约敖⒁糯昙堑妊芯浚鳵FLP研究，确定中日苑掷嗟匚唬秩夯郑匝橙禾逖≡瘢贾盅∮峁┛煽康睦砺垡谰荩苑敝成镅У难芯看蛳录崾档幕。畈刮夜谡庖涣煊虻难芯靠瞻住

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