多模式频率超声波调控玉米醇溶蛋白-壳聚糖复合凝聚层的自组装及包埋白藜芦醇机制研究

Ultrasonic technology has been proven effective at preparing protein-polysaccharide complex coacervation and improving encapsulation properties for active ingredients. The mechanism of ultrasonic influence on encapsulation properties of protein-polysaccharide complex coacervation is a hot research area of ultrasonic sonochemistry, protein biochemistry and polysaccharide biochemistry. In this project, zein-chitosan complex coacervation will be prepared to encapsulate resveratrol by multi-frequency ultrasonication. The phase behaviors and thermodynamic stability of zein-chitosan complex coacervation will be studied. The influence of multi-frequency ultrasound on particle size distribution, electrophoretic properties, turbidity and thermal stability of zein-chitosan complex coacervation will be studied systematically. Spectroscopic techniques such as near-UV circular dichroism, infrared spectroscopy, fluorescence spectroscopy, as well as scanning electron microscopy and atomic force microscopy will be employed to study the effects of multi-frequency ultrasound on the tertiary structure and surface microstructure of zein-chitosan complex coacervation. The relationships between physicochemical properties, surface microstructure, morphology of zein-chitosan complex coacervation and the encapsulation efficiency and release performance of resveratrol will also be studied. The ultimate goal was to clarify the mechanisms of muti-frequency ultrasound induced zein-chitosan complex coacervation self-assembly and the related influence on encapsulation of resveratrol. This project will help elucidating the mechanisms and help on future establishment of encapsulation methods of protein-polysaccharide complex coacervation using ultrasound.

超声波能够大规模应用于蛋白质-多糖复合凝聚层制备及显著改善其对生物活性成分包埋特性，相关机制一直是国际超声化学和蛋白质/多糖生物化学研究的热点问题。本项目拟以玉米醇溶蛋白和壳聚糖制备复合凝聚层并对白藜芦醇进行包埋，经多模式频率超声波处理，建立蛋白质-多糖自组装动力学和热力学相分离模型，系统研究超声波对玉米醇溶蛋白和壳聚糖复合凝聚层粒径分布、电荷分布、表观粘度和热力学等功能特性的影响。借助近紫外圆二色谱、红外光谱、荧光光谱和冷冻扫描电镜、原子力显微镜等技术研究超声波对复合凝聚层高级结构和表面微观结构的影响；再结合白藜芦醇包埋效率和释放性能及包埋物稳定性等宏观特征和复合凝聚层表面微观结构、形貌特征等微观信息，研究多模式频率超声波对蛋白质-多糖自组装及包埋白藜芦醇行为的影响机制。本项目涉及科学问题的解决有助于超声波强化蛋白质-多糖复合凝聚层包埋技术方法的建立及科学问题的阐述。